mB7H4-Ig融合蛋白对咪喹莫特诱导小鼠银屑病样皮损的干预作用

Effect of m B7H4-Ig fusion protein on mouse psoriasis-like lesions induced by imiquimod

目的:探讨mB7H4-Ig融合蛋白对咪喹莫特诱导小鼠银屑病的干预作用。方法:ELISA法测定高压注射重组质粒在体内的表达水平;用咪喹莫特诱导小鼠银屑病模型,治疗组高压注射mB7H4-Ig质粒而对照组注射Flag-Ig质粒,HE染色检测皮损改变,流式细胞术测定外周血免疫细胞亚群比例。结果:高压注射后小鼠体内可表达mB7H4-Ig融合蛋白;与对照组小鼠相比,治疗组小鼠皮肤的银屑病皮损炎症症状及病理改变相对较轻,PASI分数降低,外周血中的中性粒细胞比例明显降低而CD4^+T细胞比例上调。结论:mB7H4-Ig融合蛋白通过抑制炎症反应因而改善咪喹莫特诱导的小鼠银屑病皮损病变,有望成为治疗银屑病新策略。

Objective： To explore the effects of m B7H4-Ig fusion protein on mouse psoriasis-like lesions induced by imiquimod（ IMQ）. Methods： The level of m B7H4-Ig fusion protein was detected by ELISA after hydrodynamic injection of recombinant plasmids. The therapeutic groups were injected with m B7H4-Ig plasmids and control groups were injected with Flag-Ig plasmids following IMQ treatment. 5 days after treatment,the skin lesions were determined by hematoxylin-eosin staining and the percentage of immune cells in murine peripheral blood cells was measured by flow cytometric analysis. Results： The expression of m B7H4-Ig fusion protein was detected in mouse sera after hydrodynamic injection. Compared with control groups,m B7H4-Ig groups were alleviated,with decreased epidermal inflammation and lower PASI scores. The percentage of neutrophils in peripheral blood was reduced significantly in m B7H4-Ig groups,but the percentage of CD4＋T cell was increased. Conclusion： m B7H4-Ig fusion protein improved imiquimod induced psoriasis-like lesions by inhibiting the inflammatory response,indicating the potential therapeutic strategy of B7H4-Ig fusion in psoriasis.