介入热化疗栓塞治疗对肝癌患者癌细胞恶性生物学行为的影响

Effect of interventional thermotherapy embolism therapy on malignant biological behavior of cancer cells in patients with hepatocellular carcinoma

目的:研究介入热化疗栓塞治疗对肝癌患者癌细胞恶性生物学行为的影响。方法:将94例原发性肝癌患者随机分为A组和B组,每组47例,A组患者接受介入热化疗栓塞术治疗、B组患者接受常规肝动脉化疗栓塞术治疗。化疗后,采集血清样本并检测肿瘤标志物的含量,采集肝癌病灶并检测增殖基因、侵袭基因的表达量。结果:治疗后第4周、第8周时,A组和B组患者血清中AFP、AFP-L3、GP73的含量均显著低于治疗前,且A组患者血清中AFP、AFP-L3、GP73的含量均低于B组,差异有统计学意义(P<0.05);治疗后第8周时,A组患者肝癌病灶中LETM1、Cyclin D1、CDK4、BMP4、Vimentin、N-cadherin、FAT10、Rho A、ROCK的表达量低于B组,PPP1R16A、TMPy P4的表达量高于B组,差异均有统计学意义(P<0.05)。结论:介入热化疗栓塞治疗肝癌能够较肝动脉化疗栓塞术更为有效地抑制癌细胞增殖和侵袭。

Objective：To study the effect of interventional thermotherapy embolism therapy on malignant biological behavior of cancer cells in patients with hepatocellular carcinoma.Methods：primary liver cancer patients received hepatic arterial chemoembolization were enrolled and randomly divided into group A and group B.Patients in group A were treated with interventional thermochemotherapy embolization therapy,group B received routine hepatic artery chemoembolization.After chemotherapy,the serum samples were collected and the tumor markers were detected,and hepatocellular carcinoma lesion were collected and the expression of proliferative and invasive genes were detected.Results：Fourth weeks and eighth weeks after treatment,the content of serum AFP,AFP-L3 and GP73 contents of group A and group B were significantly lower than those before treatment and serum AFP,AFP-L3 and GP73 contents of group A were significantly lower than group B;Eighth weeks after treatment,the expression of LETM1,Cyclin D1,CDK4,BMP4,Vimentin,N-cadherin,FAT10,Rho A and ROCK in hepatocellular carcinoma lesion of group A were significantly lower than group B,expression of PPP1 R16 A and TMPy P4 were significantly higher than group B.Conclusion：Interventional hyperthermia embolization can effectively inhibit the proliferation and invasion of hepatocellular carcinoma compared with transcatheter arterial chemoembolization.