4-己基间苯二酚对凡纳滨对虾3种腐败菌的抑菌活性及虾品质的影响（英文）

Antibacterial Activity of 4-Hexylresorcinol against Three Spoilage Bacteria in Culture and Its Effect on the Quality of Pacific White Shrimp

为研究4-己基间苯二酚(4-hexylresorcinol,4-HR)的抑菌活性及对虾品质的影响,通过体外及接菌实验分别评价了其对来源于凡纳滨对虾的麦芽糖肉食杆菌(Carnobacterium maltaromaticum)、腐败希瓦氏菌(Shewanella putrefaciens)和杀鲑气单胞菌(Aeromonas salmonicida)的抑菌活性及其对凡纳滨对虾冷藏期间品质变化的影响。牛津杯法和抑菌生长曲线显示4-HR能够显著抑制这3种腐败菌的生长。通过对比处理前后腐败希瓦氏菌的电导率和流式细胞术分析,发现其抑菌机理可能与增强细菌膜通透性有关。将0.25 mg/m L的4-HR溶液涂膜于凡纳滨对虾上,发现在冷藏期间(4℃)凡纳滨对虾的黑变程度较低而白度值较高,表明0.25 mg/m L 4-HR能有效抑制凡纳滨对虾的黑变。但涂膜4-HR的凡纳滨对虾适冷菌总数和挥发性盐基氮值仅在贮藏的前4 d低于未涂膜的对照组;而到贮藏终点时,涂膜4-HR的凡纳滨对虾适冷菌总数较对照组高0.14(lg(CFU/g))。结果表明尽管4-HR对3种腐败菌具有良好的体外抑菌活性,但在凡纳滨对虾贮藏过程中4-HR优先作为防黑变剂抑制对虾黑变。

The antibacterial activity of 4-hexylresorcinol against the spoilage bacteria Carnobacterium maltaromaticum, Shewanella putrefaciens and Aeromonas salmonicida from Pacific white shrimps and its effect on the quality of Pacific white shrimps was assessed in this study. The results of Oxford cup method and the growth inhibition curves for the bacteria indicated that 4-hexylresorcinol showed a strong inhibitory effect on the three spoilage bacteria. The determination of electrical conductivity and flow cytometric analysis showed that 4-hexylresorcinol led to increased permeability of the cytoplasmic membrane of S. putrefaciens cells. When 0.25 mg/mL 4-hexylresorcinol was applied in shrimps, melanization was inhibited according to the low melanosis score and high whiteness value during cold storage at 4 ℃. However, a signifcant inhibitory effect on the increase of psychrotrophic bacterial counts and total volatile basic nitrogen was only observed in the frst 4 days in comparison with the control. At the end of storage, the bacterial count of 4-hexylresorcinol-treated samples was 0.14 （lg（CFU/g））, which was higher than that of the control. These results showed that though 4-hexylresorcinol was effective in inhibiting the growth of the three spoilage bacteria in vitro, it was preferred to be used as a melanosis inhibitor for shrimps rather than as an inhibitor against the growth of spoilage bacteria in shrimps.