摘要
以秀丽隐杆线虫(Caenorhabditis elegans)作为模式生物,在其生长培养基中分别添加4、40、400 mmol/L的乙醇试剂,观察乙醇对线虫寿命的调控作用。通过检测对照组和喂食乙醇溶液实验组线虫体内寿命相关基因(age-1、pdk-1、akt-1、let-363)的表达差异,探究乙醇调控线虫寿命的分子机制。结果表明,喂食400 mmol/L乙醇可有效提高线虫寿命,分别将寿命中位数和平均寿命提高了11.76%和15.78%,且与对照组相比均具有显著性差异(P<0.05)。利用实时荧光定量聚合酶链式反应检测基因表达,结果表明乙醇可能通过抑制age-1基因表达,引发胰岛素信号传导通路中一系列相应下游基因表达变化从而延长线虫寿命;雷帕霉素受体信号传导通路中let-363基因也因乙醇的摄入及胰岛素信号传导通路下游基因的抑制作用而表达下降,两者联合达到显著延长线虫寿命的作用效果。
In this paper, the effect of ethanol on Caenorhabditis elegans lifespan was investigated by culturing it in a medium with different concentrations (4, 40 and 400 mmol/L) of ethanol. The results showed that 400 mmol/L ethanol could signi?cantly extend the median lifespan by 11.76% and the mean lifespan by 15.78% (P 〈 0.05). The expression of lifespan-related genes (age-1, pdk-1, akt-1 and let-363) was detected using real-time quantitative polymerase chain reaction (qPCR) to explore the potential molecular mechanisms. Our results demonstrated that ethanol induced lifespan extension in C. elegans could be due to the suppressed expression of the above four genes and consequent regulation of the downstream genes in both the insulin/IGF signalling (IIS) and mammalian target of rapamycin (mTOR) pathways.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第21期165-169,共5页
Food Science
基金
国家自然科学基金面上项目(31071170)
BIOX研究室启动资金项目(HIT0904)
关键词
秀丽隐杆线虫
长寿
乙醇
信号传导通路
Caenorhabditis elegans
longevity
ethanol
signaling pathways