吉林人参低聚肽的抗氧化作用

Antioxidant Effect of Oligopeptide Extracted from Panax ginseng Grown in Jilin Province

目的:探讨吉林人参低聚肽(ginseng oligopeptide,GOP)对D-半乳糖过氧化损伤大鼠抗氧化作用的影响。方法:取SD大鼠90只,设立9个实验组:6个GOP剂量组(0.062 5、0.125 0、0.250 0、0.500 0、1.000 0、2.000 0 g/kg mb)、1个空白对照组、1个乳清蛋白组(0.250 0 g/kg mb)和1个模型对照组。除空白对照组外,其余各组每天腹腔注射D-半乳糖125 mg/kg mb,连续6周,造成过氧化损伤模型。造模成功后继续腹腔注射D-半乳糖并连续灌胃45 d后进行血清和肝脏氧化应激指标检测。结果:GOP可以显著降低大鼠脂质氧化产物和蛋白质氧化产物水平,显著提高大鼠抗氧化酶活力和抗氧化物质含量(P<0.05),且效果优于乳清蛋白。结论:GOP对D-半乳糖所致过氧化损伤大鼠有抗氧化作用。

Purpose： To investigate the antioxidant effect of oligopeptide extracted from Panax ginseng grown in Jilin province （GOP） on rats with D-galactose-induced oxidative damage. Methods： In total, 90 specific pathogen free （SPF） male SD rats were randomly divided into 9 groups： control group, model group, whey protein group, and six GOP groups with different doses. The rats in all groups except the control group were subjected to intraperitoneal injection of 125 mg/kg D-galactose for 6 weeks. Then, the rats in the six GOP groups were gavaged with 0.062 5, 0.125 0, 0.250 0, 0.500 0, 1.000 0 and 2.000 0 g/kg mb GOP, respectively, while those in the control and model groups were gavaged with the same volume of distilled water. The rats in the whey protein group were gavaged with 0.250 0 g/kg mb whey protein. The administration lasted for 45 days. At the same time, the rats in all groups except the control group were injected with D-galactose 125 mg/kg mb uninterruptedly. Thereafter, the oxidative stress parameters in serum and liver tissue were determined. Results： The levels of lipid peroxide and protein peroxide in the GOP groups were signi？cantly lower than those in the model group. Meanwhile, the antioxidant enzyme activities and the antioxidant levels signi？cantly increased compared with the model group （P 〈 0.05）, and the antioxidant effect of GOP was better than that of whey protein. Conclusion： GOP has an antioxidant effect on rats with D-galactose-induced oxidative damage.