补肾调经方、逍遥丸对促性腺激素预处理小鼠排卵数目的影响

Effect of Bushen Tiaojing Formula and Xiaoyao Wan on Ovulation Number of Gonadotrophins-Preprocessed Mice

目的:探讨补肾调经Ⅱ号方与Ⅲ号方高低剂量序贯使用、逍遥丸高低剂量序贯使用对促性腺激素预处理未成年小鼠排卵数目的影响。方法:17组未成年雌性小鼠腹腔注射血促性素(PMSG)、绒促性腺素(h CG)的基础上在实验第1~3 d灌服补肾调经Ⅱ号方或逍遥丸高低剂量(对照组灌服蒸馏水),实验第4~5 d灌服Ⅲ号方或逍遥丸高低剂量(对照组灌服蒸馏水)。注射h CG后19 h在体式显微镜下将输卵管壶腹部刺破,释放出新鲜卵子,拍照后计数卵子数,对卵子数进行单因素方差分析、析因分析。结果:单因素方差分析:补肾组以补肾调经Ⅱ号方高剂量与补肾调经Ⅲ号方高剂量序贯使用较对照组小鼠排卵数目增多(P=0.003),析因分析表明二者序贯使用有交互作用(P=0.047)、排卵数目增多与使用补肾调经Ⅱ号方高、低剂量有关(P=0.004)。逍遥组以逍遥丸高剂量、低剂量序贯使用较对照组小鼠排卵数目增多(P=0.007),析因分析表明二者序贯使用有交互作用(P=0.046)、排卵数目增多与排卵期使用逍遥丸高、低剂量差异显著(P=0.039)有关。结论:补肾调经Ⅱ号方使阴精盛助卵泡发育成熟而导致排卵数目增多;逍遥丸低剂量疏肝理气助阴阳转化顺利可使排卵数目多。补肾法、疏肝法分别序贯使用可提高药物疗效。

Objective： to explore the effect of sequential use of high and low dose of Bushen Tiaojing Formula（Kidney-reinforcing and Menstruation-regulating Formula） II and III,and Xiaoyao Wan on ovulation number of gonadotrophins-preprocessed immature mice. Methods： 17 groups of immature female mice were given intraperitoneal injection of pregnant mares＇ serum gonadotropin（PMSG）and human chorionic gonadotropin（hCG） before being fed with high and low dose of Formula II or Xiaoyao Wan on 1 - 3 days of the experiment,and with Formula III or Xiaoyao Wan on 4 - 5 days（control group were fed with distilled water）. After 19 hours of hCG injection,the ampulla of fallopian tube is cut under the stereomicroscope,releasing fresh ovum. Take a picture and count the ovum number which was then analyzed by one-way ANOVA and factorial analysis. Results： One-way ANOVA showed that the ovulation number of Bushen group increased after sequential use of high and low dose of Bushen Tiaojing Formula II and III,compared to control group（P = 0. 003）; factorial analysis showed that there was interaction between the two sequential use（P = 0. 047）,and the increase of ovulation number is related to high and low dose of Formula II（P = 0. 004）. The ovulation number of Xiaoyao group increased after sequential use of high and low dosage of Xiaoyao Wan（P = 0. 007）; factorial analysis showed that there was interaction between the two sequential use（P = 0. 046）,and the increase of ovulation number is related to the difference between high and low dosage of Xiaoyao Wan in ovulation period（P = 0. 039）. Conclusion： Bushen Tiaojing Formula II can enrich yin essence which contributes to follicular maturation so to increase the ovulation number; low dose of Xiaoyao Wan can sooth liver and regulate qi which contributes to yin-yang transformation so to increase the ovulation number. The sequential therapy of Bushen and Shugan（soothing liver）can improve the curative effect of the medicine.