UPLC法测定香蕉、皇帝蕉皮及其果肉中羽扇豆酮含量

Determination of Lupenone in Peel and Flesh of Musa nana Lour. and Musa acuminata cv. Mas(AA) by UPLC

目的:建立超高效液相色谱法分离和测定不同收集期的香蕉和皇帝蕉皮及其果肉中羽扇豆酮含量的方法,并优化提取条件。方法:采用甲醇回流提取法对2种蕉皮及其果肉的羽扇豆酮进行提取分离,减压浓缩,选用Waters ACQUITY UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7μm),流动相为乙腈-水(85∶15,V/V)溶液,流速0.3 mL/min,柱温30℃,检测波长206 nm。结果:香蕉和皇帝蕉果肉中均未检测出羽扇豆酮,但2种蕉皮中有较高含量的羽扇豆酮,并且在16.70~59.96 mg/100 mL质量浓度范围内有良好线性关系(r=0.999 6),羽扇豆酮平均回收率为99.00%,相对标准偏差为3.0%。结论:该法回收率高、重复性好,且具有专属性强、准确性高和简便快捷等特点,适用于香蕉、皇帝蕉皮中羽扇豆酮的含量测定。

Objective： To establish an ultra high performance liquid chromatographic（UPLC） method for determining the lupenone contents in the peel and flesh of Musa nana Lour. and Musa acuminata cv. Mas（AA） harvested at different times and to optimize the extraction conditions of lupenone. Methods： Samples were extracted with methanol by refluxing and the extracts were concentrated under reduced pressure. The analysis was performed on Waters ACQUITY UPLC BEH C18 column（2.1 mm × 50 mm, 1.7 μm）, with acetonitrile：water（85：15, V/V） solution as the mobile phase. The flow rate was 0.3 mL/min, column temperature was 30 ℃ and the detection wavelength was set at 206 nm. Results： No lupenone was detected in the flesh of Musa nana Lour. and Musa acuminata cv. Mas（AA）, but high lupenone contents were detected in the peel. A good linearity of the calibration was observed in the range of 16.70-59.96 mg/100 mL（r = 0.999 6）. The average recovery of lupenone was 99.00% and the precision expressed as relative standard deviation（RSD） was 3.0%. Conclusion： This method had the advantages of high recovery, good reproducibility, high specificity, high accuracy and simplicity. It was useful for the determination of lupenone in the peel and flesh of Musa nana Lour. and Musa acuminata cv. Mas（AA）.