超高效液相色谱-串联质谱法检测鸡血浆中头孢噻肟及其主要代谢物

Simultaneous Determination of Cefotaxime and Its Main Metabolite in Chicken Plasma by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

研究头孢噻肟(cefotaxime,CTX)在鸡血浆中代谢规律,建立同时检测血浆中CTX及其主要代谢物去乙酰头孢噻肟(desacetylcefotaxime,DCTX)的超高效液相色谱-串联质谱方法,以10 mg/kg的体质量剂量给药实验蛋鸡,并对给药后不同时间点采集的血液样品,进行CTX及其主要代谢物DCTX含量的检测。结果表明,所建方法中CTX和DCTX的检出限分别为0.07、0.14μg/L;定量限分别为0.23、0.99μg/L。回收率分别为86.7%~95.2%、89.5%~112%,相对标准偏差不大于7.7%,满足残留分析方法的要求;CTX在蛋鸡体内分布迅速,给药后即刻达到峰值(56.34 mg/L),且代谢较快,给药24 h后检测值低于3.0μg/L,而主要代谢物DCTX在给药45 min后达到峰值(10.22 mg/L),消除相对缓慢,给药24 h时检测值仍可达到54.0μg/L。

In order to study the pattern of cefotaxime metabolism in chicken plasma, an ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） method was successfully developed for the simultaneous determination of cefotaxime and its metabolite desacetylcefotaxime. Cefotaxime at a dose of 10 mg/kg was administered to laying hen, and chicken plasma was collected at different time points to quantitate cefotaxime and desacetylcefotaxime by UPLC-MS/MS. The results showed that the limits of detection（LOD） for cefotaxime and desacetylcefotaxime were 0.07 and 0.14 μg/L, respectively; the limits of quantification（LOQ） were 0.23 and 0.99 μg/L, respectively. The recoveries of cefotaxime and desacetylcefotaxime ranged from 86.7%–95.2% and 89.5%–112%, respectively, with relative standard deviation（RSD） less than 7.7%, which met the requirements for quantitative analysis. Cefotaxime was rapidly distributed in laying hens and reached the peak（56.34 mg/L） immediately after administration, and the rate of cefotaxime metabolism was fast, which was detected at a level lower than 3.0 μg/L after 24 hours. The main metabolite deacetylcefetaxime reached its peak（10.22 mg/L） after 45 min, and it was eliminated at a relatively slow rate was and was still as high as 54.0 μg/L at 24 h after administration.