摘要
采用基于气相色谱-质谱联用技术的代谢组学方法 ,研究不同致病性青枯雷尔氏菌诱导番茄植株代谢物变化的异质性。将青枯雷尔氏菌强致病力菌株FJAT-91和无致病力菌株FJAT-1458分别单独接种和混合接种番茄植株,以清水为对照,接种后6、24、48、72、96h取样,测定番茄植株代谢产物的变化。结果表明,检测到的代谢物种类主要为醇类、酯类、酸类、醛类、吡啶类和烷烃类。不同处理番茄代谢产物组成变化的时间动态结果表明,FJAT-91单独接种处理48h、FJAT-1458单独接种处理48h、同时接种FJAT-91和FJAT-1458处理96h及对照处理72、96h的番茄中检测到代谢物种类最多,分别为12、18、15和15种。邻苯二甲酸二丁酯在不同处理不同时间的番茄中均检测到,为完全分布类型,其他代谢物为不完全分布类型。FJAT-91单独接种处理能诱导番茄代谢产物棕榈酸消亡,而FJAT-1458单独接种或与菌株FJAT-91混合接种及对照处理的番茄植株该代谢物维持在相当含量,说明该代谢物可能与植株免疫抗病有关。主成分分析表明,不同接种处理诱导番茄植株的代谢谱存在一定的差异,主成分一和主成分二基本上能将其区分开来。
Gas chromatography-mass spectrometry was employed for the investigation on the heterogeneity of metabolites in tomato plants infected by strains of Ralstonia solanacearum with varied pathogenicity.The plants were inoculated with the virulent FJAT-91 and/or avirulent FJAT-1458,as well as water as control.After 6,24,48,72 and 96 h,metabolites in the plants were analyzed.The results showed the metabolites were mostly alcohols,esters,acids,aldehydes,pyridines,and alkanes.The compositional differences on the plants infected by different pathogenic strains were compared.It was found that the number of metabolite varieties in the plants varied by the treatments and time.The tomato plants treated by FJAT-91 for 48 hhad 12 different metabolites;those treated by FJAT-1458 for 48 h,18;those treated by both strains simultaneously for 96 h,15;and,control treated for 72 or96 h,15.Dibutyl phthalate was the only metabolite present in all samples.n-hexadecanoic acid was absent in the plants inoculated with FJAT-91,but was detected at considerable amounts in those infected with FJAT-1458 or combined strains,and control.The discrepancy might be due to a variation in the immunology of the tomato plants.A principal component analysis on the metabolites revealed discernible differences among the tomato plants that received different treatments.It suggested that the viral infections could be identified by comparing the two most abundant metabolites in a plant.
出处
《福建农业学报》
CAS
北大核心
2017年第8期885-890,共6页
Fujian Journal of Agricultural Sciences
基金
国家公益性行业(农业)科研专项(201303015)
福建省自然科学基金项目(2015J01103)
福建省科技计划项目--省属公益类科研院所基本科研专项(2014R1018-8)
关键词
番茄
青枯病
青枯雷尔氏菌
GC-MS
tomato
bacterial wilt disease
Ralstonia solanacearum
GC-MS