玉米优良自交系成熟胚愈伤组织诱导

Research on Callus Induction from Mature Embryos of Maize Inbred Lines

玉米是重要的粮食作物和饲料作物,建立稳定、高效的玉米转化体系无疑具有重要的理论意义和应用价值。试验对自交系492、自交系26及自交系758的成熟胚愈伤组织诱导和植株再生的条件进行了摸索。结果表明在诱导培养基中添加4mg/L的2,4-D,可显著提高成熟胚愈伤组织诱导率;在继代培养基中添加10mg/L的AgNO_3、150mg/L的L-半胱氨酸,玉米成熟胚的胚性愈伤组织诱导率高。

Maize（ Zea Mays L.） is a widely grown cereal and the most important fodder crop in the world today. It is significant in theory and application to establish an efficient and stable genetic transformation system of maize. The mature embryos callus induction and plant regeneration conditions were explore of inbred line 492,26,758. The results showed that the maize mature embryo callus induction was higher when induction medium supplemented with 4 mg/L of 2,4-D The maize mature embryo callus induction was higher when subculture medium supplemented with 10 mg/L of AgNO_3,150 mg/L of L-cysteine.