不同鸡品种BNK基因序列比较分析

Comparative analysis of BNK gene sequences in different chicken breeds

BNK(C-type lectin-like receptor 2)基因为C型凝结素受体基因中的抑制性基因,与禽类先天免疫性能关系密切。本研究以NCBI中Gallus gallus BNK基因序列为参考,利用MEGA6软件对24个鸡品种的BNK基因进行序列比较分析,筛选出外显子和内含子上的SNPs位点、Indels以及氨基酸变化,并对24个鸡品种进行系统聚类。研究发现,SNPs位点大多发生在Exon3、Exon5和Exon6上,且多为错义突变。内含子上的SNPs位点大多发生在Intron1、Intron2和Intron4上。插入缺失发生在Intron2、Intron4和Exon5上,并且大多数品种Indels序列基本一致,国内外鸡品种之间并无显著差异。系统聚类分析将这些鸡品种分为3类,狼山鸡、罗斯鸡、隐性白羽肉鸡、茶花鸡和如皋黄鸡为一类;其次,雪山鸡、安卡鸡、斗鸡和萧山鸡聚为一类;固始鸡单独为一类,表明固始鸡的BNK基因为单独起源。综上表明,BNK基因在我国地方鸡品种中具有丰富的遗传多样性,与不同鸡品种的先天免疫性能存在关联,这为家禽的免疫遗传学和抗病育种提供了参考依据。

The BNK（ C-type lectin-like receptor 2） gene is an inhibitory receptor expressed on natural killer cells,which is closely related to the innate immune function of poultry. In this study,according to NCBI 5. 0 Gallus gallus,we used MEGA6 software to analyze BNK gene in 24 types of chickens. We found several SNPs,Indels and amino acids on exons and introns. In addition,we performed the clustering in 24 chickens via MEGA6. The results showed that most of the SNPs sites occurred on Exon3,Exon5 and Exon6,and most of them were missense mutations. The SNPs sites mostly occurred on Intron1,Intron2 and Intron4. And then the Indels mostly occurred on Intron2,Intron4 and Exon5. There was no significant difference between domestic and foreign chicken breeds,and most of the Indels sequences were basically the same. By cluster analysis the chickens were divided into three groups,Langshan chicken,Ross chicken,Recessive white chicken,Chahua chicken and Rugao yellow chicken as a class; Xueshan chicken,Anka chicken,Dou chicken and Xiaoshan chicken as a class; Gushi chicken as a class. According to clustering analysis,BNK gene of Gushi chicken was a single origin. In conclusion,BNK gene has abundant genetic diversity in Chinese chicken breeds and there was close relation in innate immunity with different chicken breeds,which providereference for immune genetics and disease resistance breeding of poultry.