孕早期七氟烷母体麻醉对子代大鼠认知功能的影响及其机制

Effects of early sevoflurane maternal anesthesia on cognitive function in offspring rats and its mechanism

目的研究孕早期七氟烷母体麻醉对子代大鼠认知功能的影响,并对相关机制进行初步探讨。方法将27只7周龄SD雌性大鼠随机分为实验组-1(n=9)、实验组-2(n=9)和对照组(n=9)。于孕5~7 d时进行麻醉处理,实验组-1,实验组-2分别持续吸入1.8%七氟烷4,8 h,对照组仅吸入氧气8 h,氧流量均为2 L·min-1。每组均随机取6只母鼠所产子代SD大鼠,待出生后第30天开始进行Morris水迷宫系统测定其认知功能,用逆转录聚合酶链式反应(RT-PCR)及免疫组化法检测海马组织NR2B mRNA和HGN mRNA及蛋白表达情况。结果 Morris水迷宫系统测定结果显示,3组逃避潜伏期、穿越平台次数及在平台象限停留时间差异无统计学意义(P>0.05)。实验组-1NR2BmRNA和HGN mRNA分别为0.79±0.11,0.66±0.08,实验组-2NR2BmRNA和HGN mRNA分别为0.77±0.09,0.65±0.07,对照组NR2B mRNA和HGN mRNA分别为0.78±0.08,0.68±0.07。3组NR2B mRNA和HGN mRNA表达结果差异无统计学意义(P>0.05)。实验组-1、实验组-2及对照组NR2B蛋白阳性细胞的平均灰度值分别为99.68±17.68,97.62±18.63,101.38±15.52,HGN蛋白阳性细胞的平均灰度值分别为86.49±22.04,89.14±20.15,92.15±22.51,3组子代SD大鼠海马NR2B和HGN蛋白表达量差异无统计学意义(P>0.05)。结论孕早期七氟烷母体麻醉对子代SD大鼠海马NR2B mRNA和HGN mRNA表达及NR2B蛋白表达无影响,是否与七氟烷麻醉剂量相关尚需进一步研究。

Objective To investigate the effect of sevoflurane maternal anesthesia on cognitive function in offspring rats, and to explore the mechanism. Methods A total of 27 SD female rats aged 7 weeks were randomly divided into test group - 1 （ n = 9 ）, test group - 2 （ n = 9） and control group （n = 9）. At 5 - 7 d after pregnancy, test group - 1, test group-2 were inhaled 1.8% sevoflurane for 4, 8 h, control group only inhaled oxygen for 8 h, oxygen flow rate 2 L · min- 1 Each group was randomly selected 6 offspring rats, and the morris water maze system was used to determine its cognitive function on the 30 d after birth, the ex- pression of NR2B mRNA and HGN mRNA in hippocampus were detected by reverse transcription polymerase chain reaction （RT- PCR）, the expression of NR2B protein in hippocampus was detected by immunohistochemistry.Results Morris water maze system showed that there was no significant difference in three groups in the escape laten- cy, the number of crossing the platform and the time of residence in the platform quadrant. The NR＇2B mRNA and HGN mRNA in test group - 1 were 0.79 ± 0. 11, 0. 66 ± 0.08. The NR2B mRNA and HGN mRNA in test group - 2 were 0. 77 ± 0.09, 0. 65 ± 0. 07. The NR2B mRNA and HGN mRNA in control group were 0. 78 ± 0. 08, 0. 68 ± 0. 07, with no significant difference in three groups （ P 〉 0. 05 ）. The average gray values of NIL2B protein positive cells in test group - 1, test group - 2 and control group were 99.68 ± 17.68, 97.62 ± 18.63, 101.38 ± 15.52. The average gray values of HGN protein positive cells in test group - 1, test group -2 and control group were 86.49 ± 22.04, 89. 14 ±20. 15, 92. 15 ± 22.51, with no significant difference in three groups （ P 〉 0. 05 ）. Conclusion Early pregnancy sevoflurane maternal anesthesia has no affect in the expression of NR2B mRNA and HGN mRNA and NR2B protein in hippocampus of offspring SD rats, whether it is related to the dose of sevoflurane anesthesia need further study.