武汉地区丙型肝炎病毒基因型分布及其少见基因亚型系统进化树分析

Distribution of hepatitis C virus genotype and phylogenetic tree analysis of its rare sub-genotypes in Wuhan

目的研究武汉地区丙型肝炎病毒(HCV)基因型分布情况,构建HCV少见基因亚型系统进化树。方法收集武汉地区258例慢性丙型肝炎患者血浆样本,采用基于HCV非结构蛋白5B(NS5B)区的Sanger测序法对患者HCV进行基因分型,将HCV NS5B区测序结果与NCBI Genotyping tool中HCV基因型数据库进行比对,得到受检患者的HCV基因亚型,采用MEGA5.0软件对武汉地区HCV少见基因亚型进行系统进化树分析。结果 258例丙型肝炎患者样本均被成功分型,共检出6种基因亚型:1b型(71.32%)、2a型(20.16%)、6a型(3.49%)、3b型(2.71%)、3a型(1.16%)、1a型(1.16%)。武汉地区HCV少见基因亚型系统进化树被成功构建。结论武汉地区HCV优势基因亚型为1b型和2a型,6a型、3a型、3b型、1a型较少见。武汉地区3例3a型HCV可能由日本传入;大部分3b型HCV可能由越南传入,NO.379样本出现较大变异;6a型HCV可能由越南传入。

ObjectiveTo study the distribution of hepatitis C virus（HCV） genotype in Wuhan,and to analyze the phylogenetic tree of rare HCV sub-genotypes. MethodsPlasma samples were collected from 258 patients with hepatitis C in Wuhan. Sanger sequencing based on HCV-nonstructural protein 5B（NS5B） was used to genotyping. The results were compared with the genotypes by NCBI Genotyping tool. The HCV sub-genotypes were obtained. The phylogenetic tree of rare HCV sub-genotypes was analyzed by MEGA5.0 software. ResultsHCV sub-genotypes were successfully identi？ed in all patients,and 6 HCV sub-genotypes,including type 1b（71.32%）,type 2a（20.16%）,type 6a（3.49%）,type 3b（2.71%）,type 3a（1.16%） and type 1a（1.16%）,were determined. The phylogenetic tree of rare HCV sub-genotypes was constructed successfully in this region. Conclusions The dominant HCV sub-genotypes are type 1b and type 2a,and type 6a,type 3a,type 3b and type 1a are rare in Wuhan. HCV sub-genotype 3a may be imported from Japan. Most of type 3b may be imported from Vietnam, and sample 379 appears large variation. Type 6a may be imported from Vietnam.