摘要
目的探讨外周血胸腺嘧啶核苷激酶1(TK1)和S期细胞百分比(SPF)在急性髓系白血病(AML)诊断、疗效监测及预后中的意义。方法选取AML患者58例、血液系统良性增殖疾病患者(良性增殖组)6例和体检健康者(正常对照组)20名,采用印迹免疫-增强发光法检测TK1,采用流式细胞术(FCM)检测SPF。58例AML患者经1个疗程的标准诱导治疗后,比较治疗前、缓解组(39例,其中完全缓解11例、部分缓解28例)和未缓解组(19例)的TK1和SPF;同时检测58例AML患者治疗后1周和1个月的TK1和SPF。结果 AML组治疗前TK1为(2.43±1.02)pmol/L、SPF为(4.62±2.33)%,明显高于良性增殖组[TK1为(1.12±0.41)pmol/L、SPF为(2.62±1.05)%]及正常对照组[TK1为(0.39±0.15)pmol/L、SPF为(2.21±0.96)%](P<0.05)。AML完全缓解组TK1为(0.78±0.44)pmol/L、SPF为(1.89±1.23)%,明显低于治疗前和未缓解组[TK1为(4.26±0.46)pmol/L、SPF为(5.58±3.12)%](P<0.05)。AML患者治疗1个月后,TK1和SPF分别为(1.97±0.89)pmlol/L和(2.89±1.03)%,明显低于治疗前(P<0.05)。结论外周血TK1联合SPF检测能更准确地预测AML的细胞增殖情况,可很好地指示AML的治疗及提示预后。
ObjectiveTo evaluate thymidine kinase 1(TK1) and S-phase fraction(SPF) in peripheral blood for the diagnosis,treatment monitoring and prognosis of acute myeloid leukemia(AML). MethodsA total of 58 patients with AML were enrolled,and 6 patients with benign proliferative disease in hematological system and 20 healthy subjects were enrolled as well. TK1 levels were determined by dot-blot enhanced chemiluminescence,and SPF were calculated by ?ow cytometry. After a standard inductive treatment,the levels of TK1 and SPF were compared in remission group(39 cases,including 11 cases of complete remission and 28 cases of partial remission) and non-remission group(19 cases) with those before treatment. The levels of TK1 and SPF were determined at the time points of a week and a month after treatment. ResultsThe levels of TK1 [(2.43±1.02)pmol/L] and SPF[(4.62±2.33)%] in AML group before treatment were higher than those in benign proliferative disease group [(1.12±0.41)pmol/L and(2.62±1.05)%] and healthy control group[(0.39±0.15)pmol/L and(2.21±0.96)%](P〈0.05). The levels of TK1 [(0.78±0.44) pmol/L] and SPF [(1.89±1.23)%] decreased in complete remission group compared with those before treatment and in non-remission group [(4.26±0.46)pmol/L and(5.58±3.12)%](P〈0.05). After a standard inductive treatment for a month,the levels of TK1 [(1.97±0.89)pmol/L] and SPF [(2.89±1.03)%] were lower than those before treatment(P〈0.05). ConclusionsThe combined determination of TK1 and SPF is accurate for the proliferation status of AML and can indicate treatment and prognosis status of AML.
出处
《检验医学》
CAS
2017年第10期59-62,共4页
Laboratory Medicine
基金
武警后勤学院附属医院资助项目(FYM201615)