摘要
中东呼吸综合征冠状病毒(Middle East respiratory syndrome coronavirus,MERS-CoV)2012年在中东地区的大暴发及其快速传播能力,已成为了全球科研工作者关注的重点,寻找有效的治疗和预防措施对MERS-CoV感染防治起着至关重要的作用.临床研究证实,病毒感染者恢复期血清中含有大量的中和抗体,并具有有效的临床治疗作用,说明中和抗体能有效、安全的作为病毒感染治疗手段.纤突(spike,S)蛋白是介导MERS-CoV感染宿主细胞的关键结构蛋白,是目前此病毒中和性单抗研究的关键位点.本文综述了MERS-CoV S蛋白结构特点、中和性单抗制备免疫原类型及不同种类中和性单抗的制备方式,并结合当前研究现状展望了MERS-CoV中和单抗研发前景.
The rapid spread and outbreak of Middle East respiratory syndrome coronavirus ( MERS-CoV) in the regions of Middle East in 2012 have been a great concern for researchers worldwide. Thus, efficient preventative and therapeutic countermeasures are urgently needed. Clinical studies have con-firmed that high titers of neutralizing antibodies ( Abs) against MERS-CoV in patients during convalescence have protective potency, which indicates that neutralizing Abs are safe and effective for the treatment of MERS-CoV infection. Spike ( S) protein is a key structural protein that mediates MERS-CoV infection and currently a critical protein for studying MERS-CoV neutralizing monoclonal antibodies. This review summari-zes recent advances in identifying neutralizing McAbs against MERS-CoV through describing the structural characteristics of MERS-CoV S protein, different kinds of MERS-CoV vaccines and methods for mAbs screening. Furthermore, we propose the prospects for future research on MERS-CoV neutralizing McAbs ac-cording to the current research progress.
作者
陈迎珠
秦堃
谭文杰
Chen Yingzhu Qin Kun Tan Wenjie(School of Laboratory Medicine and Life Sciences, Institute of Medical Virology, Wenzhou Medical University, Wenzhou 325000, China National Institute for Viral Disease Control and Pre- vention, Chinese Center for Disease Control and Prevention, Beifing 102206, China)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2017年第9期696-702,共7页
Chinese Journal of Microbiology and Immunology
基金
国家重点研发计划(2016YFD0500300,2016YFCl200900)
国家传染病重大专项“十三五”计划(2016ZXl0004001-003).