宽叶金粟兰中具抗乳腺癌转移活性的倍半萜类化学成分研究

Sesquiterpenes with anti-metastasis breast cancer activity from Chloranthus henryi

利用多种色谱分离方法和波谱学鉴定方法对宽叶金粟兰乙酸乙酯部位的化学成分进行了分离鉴定,并借助药理学方法对其中得到的部分化合物进行了抗乳腺癌转移活性的初步筛选。总共从宽叶金粟兰乙酸乙酯部位中分离得到15个倍半萜及其二聚体类化合物,分别鉴定为zedoarofuran(1),chlorajapolide D(2),4β,8β-dihydroxy-5α(H)-eudesm-7(11)-en-8,12-olide(3),curcolonol(4),lasianthuslactone A(5),chlomultin C(6),(1E,4Z)-8-hydroxy-6-oxogermacra-1(10),4,7(11)-trieno-12,8-lactone(7),shizukanolide E(8),shizukanolide F(9),9α-hydroxycurcolonol(10),shizukaol B(11),shizukaol C(12),cycloshizukaol A(13),sarcandrolide B(14),henriol A(15)。其中化合物2,8~10为首次从该属植物中分离得到,化合物2,5,8~10,12,14为首次从该植物中分得。并对所分得量较大的5个化合物进行了抗乳腺癌转移活性筛选,其中化合物4,11,12表现出较强的活性。

To study sesquiterpenes with anti-metastasis breast cancer activity from Chloranthus henryi, ten sesquiterpenes ,zedoaro- furan （1）, chlorajapolide D （2）, 4β, 8β-dihydroxy-5α（H）-eudesm-7（ll）-en-8, 12-olide （3）, curcolonol （4）, lasianthuslactone A （5）, chlomultin C （6）, （ 1E,4Z）-8-hydroxy-6-oxogermacra-I （ 10）, 4, 7 （ 11 ） -trieno-12, 8-1actone （7）, shizukanolide E （8） , shizukanolide F （9） , 9α-hydroxycurcolonol （10）, and five bis-sesquiterpenes, shizukaol B （11）, shizukaol C （12） , cycloshizukaol A （13） , sarcandrolide B （14） , henriol A（15）, were isolated by using different kinds of column chromatography methods from the ethyl acetate part of Ch. henryi and their structures were identified based on spectroscopic methods. Compounds 2, 8, 9, and 10 were obtained from the genus Chloranthus for the first time. Compounds 2, 5, 8-10, 12, and 14 were obtained from this plant for the first time. Some isolated compounds were subjected to evaluate the anti-metastasis breast cancer activity by using pharmacological methods, and only compounds 4, 11, and 12 were potent active.