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基于等温滴定量热技术探究黄芩质量表征新方法 被引量:4

New qualities control strategy of Scutellaria baicalensis by isothermal titration calorimetry
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摘要 黄芩黄连药对水煎自沉淀现象已被广泛关注,产生自沉淀现象的本质是药对中酸碱性活性成分相互作用形成难溶性分子复合物,本试验设计基于中药配伍后有效成分间的相互作用,探索将等温滴定量热技术(isothermal titration calorimetry,ITC)应用于表征黄芩药材质量的可行性,建立以活性成分群为指标衡量药材质量的新方法。以不同批次黄芩药材(包括市售的3批黄芩药材和市场上常用做以次充好的黄芩苗)作为研究对象,应用ITC分别测定黄连中代表性功效成分小檗碱(2.5mmol·L^(-1))与不同批次黄芩水煎液间相互作用能量值,将小檗碱滴定空白水溶液的能量变化(2.51μJ)作为空白对照,结果显示以小檗碱为标准物质,滴定不同批次黄芩水煎液中黄芩苷类成分的热量变化由高到低依次为黄芩A(-317.20μJ)、黄芩B(-292.83μJ)、黄芩C(-208.95μJ)、黄芩苗(-21.53μJ)。其中小檗碱滴定黄芩苗的热量变化显著低于3批黄芩药材。应用紫外分光光度法以黄芩苷计测定不同批次黄芩水煎液在最大吸收波长处的吸光度值。紫外测定结果显示,相同稀释条件的不同批次药材吸光度由高到低依次为黄芩A(0.372)、黄芩B(0.333)、黄芩C(0.272)、黄芩苗(0.124),且黄芩苗吸光度显著低于前三组。ITC滴定与紫外测定结果相吻合,证明ITC能用于表征黄芩药材质量,基于ITC建立的以一类活性成分为指标评价黄芩药材质量的方法是简便、科学且可行的,此试验为构建更加全面、系统地表征药材质量提供了新思路。 It has been focused on that there will be precipitates when decoction of Scutellariat Radix mixed with Coptidis Rhizoma. Precipitation was derived from interaction between acidic and basic compounds. This study was based on the interaction between active ingredients after compatibility, strived to explore whether it was feasible to judge the qualities of different Scutellariat Radix by isother- mal titration calorimetry ( ITC), build a new method established to characterize the qualities of traditional Chinese medicine by taking a series of active ingredients as index. We selected Scntellariat Radix ( including three batches of different Scutellariat Radix bought from market and immature Scutellariat Radix which usually was used as adulterant) in different batches as the samples. First, we used ITC to determine the binding heat of the reactions between berberine and the decoctions of different Scntellariat Radix. The test showed that the binding heat of berberine titrated Scutellariat Radix was Scutellariat Radix A ( -317.20 p.J), Scutellariat Radix B ( -292. 83 μJ), Scutellariat Radix C ( -208.95 μJ) and immature Scutellariat Radix ( -21.53 μJ), respectively. We chose deionized water ti- trated by berberine (2. 51 μJ) as control. The heat change of berberine titrated immature Scutellariat Radix was much less than berber- ine titrated Scutellariat Radix. Then we determined the absorbance of different decoctions of Scutellariat Radix by UV Spectropbotome- try on the maximum absorption wavelength, and the result is : Scutellariat Radix A (0. 372), Scutellariat Radix B (0. 333 ), Scutellar- iat Radix C (0. 272) , immature Scutellariat Radix (0. 124). The absorbance of immature Scutellariat Radix was also less than Scutel- lariat Radix. The result of ITC assay was corresponded to UV spectrophotometry test. In conclusion, ITC could be used to characterize the quality of Scutellariat Radix. The new method to characterize the qualities of traditional Chinese medicine by taking a kind of active ingredients as index building by ITC was simple, scientific and feasible.
作者 李桐 张昊 田学浩 王辉 任广喜 荆文光 徐冰 褚福浩 王鹏龙 雷海民 LI Tong ZHANG Hao TIAN Xue-hao WANG Hui REN Guang-xi JING Wen-guang XU Bing CHU Fu-hao WANG Peng-long LEI Hai-min(School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China China National Traditional Chinese Medicine Corporation, Beijing 100195, China)
出处 《中国中药杂志》 CAS CSCD 北大核心 2017年第20期3969-3973,共5页 China Journal of Chinese Materia Medica
基金 国家自然科学基金项目(81603256) 北京中医药大学中青年教师面上项目(2015-JYB-JSMS023) 北京中医药大学创新团队资助项目(2011-CXTD-15) 北京市中药新药与物质基础研究重点试验室和国家级大学生创新创业训练计划项目(201710026044)
关键词 等温滴定量热 分子相互作用 紫外分光光度法 黄芩 质量 isothermal titration calorimetry (ITC) UV spectrophotometry molecular interaction Scutellaria baicalensis qualitycontrol of traditional Chinese medicine
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