超滤膜法提取水相中茶皂素的研究

Study on extraction of tea saponin by ultrafiltration from the aqueous phase

本文以乙醇水提法提取油茶籽油后产生的水相副产物为原料,利用超滤膜法对其中丰富的茶皂素进行分离提纯。主要探索了超滤膜材料、膜孔径、茶皂素浓度、水相p H以及超滤后期加水量对茶皂素透过率以及纯度的影响。结果表明:采用截留分子量为10 k的改良纤维素复合膜(PLCGC)进行超滤,同时将水相稀释4倍至茶皂素浓度为13.13 mg/m L,p H调节至7.0,超滤后期加3倍体积的水恒容超滤,茶皂素透过率可达64.39%,纯度达84.16%。对纯化后的茶皂素进行红外光谱及液质分析,证明超滤法可以有效分离提取水相中茶皂素,实现提油后水相副产物的回收利用。

This article mainly aimed at the aqueous phase obtained from the extraction of the camellia seed oil with ethanol solution and studied the separation of tea saponin by uhrafiltration method.Material of ultrafiltration membrane,the aperture of the membrane,the initial concentration of tea saponin, the pH of the aqueous phase and the amount of water added in later period of ultrafihration were studied to explore their effects on the yield and purity of the tea saponin.The results showed that when 10 k membrane of PLCGC material was used and diluting the aqueous phase to 4 times（ 13.13 mg/mL tea saponin）, adjusting the aqueous phase pH as 7.0 and adding 3 times water during later ultrafihration period, the transmittance ratio and purity could reach 64.39% and 84.16% .Characterize the final purified tea saponin by IR and LC-MS.The result showed the tea saponin was in a high purity and it basically conformed to the features of standard substance.Therefore, ultrafihration was an effective method to separate the tea saponin from the aqueous phase.