摘要
目的:观察TNF-α刺激前后,NOX2基因在肺泡Ⅱ型上皮A549细胞的表达情况;探讨NF-κB与NOX2基因的相关性。方法:预转染NF-κB siNRA,以TNF-α刺激肺泡Ⅱ型上皮A549细胞,采用RT-PCR及免疫印迹法分别检测NOX2mRNA及蛋白的表达水平。结果:以TNF-α刺激A549细胞可观察到NOX2mRNA及NOX2蛋白表达增加(P<0.05);预转染NF-κB siRNA,NOX2mRNA及NOX2蛋白表达降低(P<0.05)。结论:TNF-α可诱导NOX2基因在A549细胞表达上调,预先沉默NF-κB可下调上述表达趋势,提示NF-κB与NOX2基因表达存在相关性。
Objective:To investigate the expression of NOX2 gene in A549 cells induced by TNF-α,and relationship between NF-κB and NOX2. Method: NF-κB siRNA was transfeeted into A549 cells and stimulated by TNF-α, RT-PCR and Western blotting were performed to analyze the expression of NOX2 gene. Result: TNF-α significantly up-regulated the transcription and translation of NOX2 gene, NF-κB RNAi could down-regulate the expression of NOX2 (P〈 0.05). Conclusion:The expression of NOX2 gene could increase in A549 cells stimulated by TNF-α,NF κB silencing could down-regulate the expression of NOX2. Our study suggested that there is a correlation between NF-κB and NOX2 gene expression.
作者
吴炜景
张家敏
李立平
WU Weijing ZHANG Jiamin LI Liping(Department of Respiratory and Critical Care Medicine, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China Department of Respiratory Medicine, Guangzhou General Hospital of Guangzhou Military Command)
出处
《临床急诊杂志》
CAS
2017年第9期682-685,共4页
Journal of Clinical Emergency
基金
国家自然科学基金资助项目(No:81541002)
福建省自然科学基金计划项目(No:2015J0102)
福建省卫生厅青年科研课题资助项目(No:2013-2-51)
福建省中青年教师教育科研项目(No:JB13085)
泉州市科技项目(No:Z[2013]0206)
福医大附二院苗圃基金(No:MP2013MP23)