TNF-α诱导A549细胞中NF-κB与NOX2的相关性研究

The association of NF-κB and NOX2 in A549 cells induced by TNF-α

目的:观察TNF-α刺激前后,NOX2基因在肺泡Ⅱ型上皮A549细胞的表达情况;探讨NF-κB与NOX2基因的相关性。方法:预转染NF-κB siNRA,以TNF-α刺激肺泡Ⅱ型上皮A549细胞,采用RT-PCR及免疫印迹法分别检测NOX2mRNA及蛋白的表达水平。结果:以TNF-α刺激A549细胞可观察到NOX2mRNA及NOX2蛋白表达增加(P<0.05);预转染NF-κB siRNA,NOX2mRNA及NOX2蛋白表达降低(P<0.05)。结论:TNF-α可诱导NOX2基因在A549细胞表达上调,预先沉默NF-κB可下调上述表达趋势,提示NF-κB与NOX2基因表达存在相关性。

Objective：To investigate the expression of NOX2 gene in A549 cells induced by TNF-α,and relationship between NF-κB and NOX2. Method： NF-κB siRNA was transfeeted into A549 cells and stimulated by TNF-α, RT-PCR and Western blotting were performed to analyze the expression of NOX2 gene. Result： TNF-α significantly up-regulated the transcription and translation of NOX2 gene, NF-κB RNAi could down-regulate the expression of NOX2 （P〈 0.05）. Conclusion：The expression of NOX2 gene could increase in A549 cells stimulated by TNF-α,NF κB silencing could down-regulate the expression of NOX2. Our study suggested that there is a correlation between NF-κB and NOX2 gene expression.