iRoot FM对牙髓卟啉单胞菌抗菌效果评价

Antimicrobial effect of a new bio-ceramic material iRoot FM on Porphyromonas endodontalis

目的:比较iRoot FM与传统根管消毒材料氢氧化钙[Ca(OH)_2]及三联抗生素糊剂(triple antibiotic paste,TAP)对牙髓卟啉单胞菌(Porphyromonas endodontalis,P.endodontalis)的抗菌效果,评价iRoot FM的抗菌性能,为临床上根管消毒材料的选择提供参考。方法:采用国际标准菌株P.endodontalis ATCC35406进行琼脂平板扩散实验。实验组为iRoot FM组、Ca(OH_)2组及TAP组,以蒸馏水作为空白对照组。将冻干的P.endodontalis复苏,増菌,接种于培养皿中,干燥后打孔,将材料分别加入小孔中,37℃厌氧箱培养72 h,测定抑菌环直径(mm)。实验重复6次(n=6),采用SPSS17.0软件包对数据进行统计学分析。结果:iRoot FM、Ca(OH)_2及TAP 3组抑菌环直径分别为(20.74±4.35)mm、(24.89±3.84)mm和(34.51±1.20)mm。其中,iRoot FM组与Ca(OH)_2组相比,无显著差异(P>0.05);而iRoot FM组、Ca(OH)_2组与TAP组相比,差异具有显著性(P<0.05)。对照组无抑菌环出现。结论:新型生物陶瓷材料iRoot FM在体外实验中对牙髓卟啉单胞菌具有较强的抗菌作用,提示其在根管消毒中具有良好的应用前景。

PURPOSE： To compare the antimicrobial effect of a new bio-ceramic material iRoot FM with traditional intracanal medicaments including calcium hydroxide [Ca（OH）2] and triple antibiotic paste （TAP）, on Porphyromonas endodontalis （P. endodontalis）, and to evaluate the antimicrobial activity of iRoot FM, providing reference for clinical use of intracanal medicaments. METHODS： P. endodontalis ATCC 35406 were used in this study. The study was divided into 3 experimental groups including iRoot FM, Ca（OH）2 and TAP group. Sterile water was used as blank control. Frozen P. endodontalis were seeded and grown overnight in the liquid medium, then P. endodontalis were seeded on BHI-blood agar plates. After the plates were dried, the materials were filled on the plates which were made by a punching machine. Zones of inhibition （mm） were measured after 72 h of anaerobic incubation at 37℃. The experimental data were analyzed statistically using SPSS 17.0 software package. RESULTS： The zones of inhibition in the 3 experimental groups： iRoot FM, Ca（OH）2 and TAP group were （20.74±4.35）mm, （24.89±3.84）mm and （34.51±1.20）mm, respectively. The zones of inhibition of the iRoot FM group and Ca（OH）2 group were significantly smaller compared with the TAP group （P〈0.05）, while there was no significant difference between the iRoot FM group and Ca（OH）2 groups （P〉0.05）. There were no zone of inhibition in the control group. CONCLUSIONS： As a new bio-ceramie material, iRoot FM shows a good antimicrobial activity against P. endodontalis and may be a promising intracanal material.