顽固性复发性阿弗他溃疡患者外周血Th17细胞与免疫球蛋白及补体水平的变化

Alteration of Th17 cells,immunoglobins and complements levels in peripheral blood of patients with refractory recurrent aphthous ulcer

目的:探讨Th17细胞与免疫球蛋白及补体在顽固性复发性阿弗他溃疡(顽固性RAU)患者外周血中的变化。方法:收集30例顽固性RAU患者和21例健康志愿者的外周血,采用流式细胞术检测Th17细胞的数量;采用免疫散射比浊法检测患者外周血血清免疫球蛋白Ig A、Ig G、Ig M、Ig E和补体C3、C4的水平,分析Th17细胞水平的改变以及Th17细胞与血清体液免疫水平之间的关系。采用SPSS17.0软件包对数据进行统计学分析。结果:顽固性RAU患者外周血Th17细胞(CD3+CD8-IL-17+)占CD4+T细胞的百分率显著高于健康对照组(P<0.01),免疫球蛋白Ig G、Ig A与Ig E的水平显著高于健康对照组(P<0.05),Ig M和补体C3、C4的水平与健康对照组相比无显著差异(P>0.05);Th17细胞与Ig A的表达呈明显的负相关(r=-0.477,P<0.01)。结论:顽固性RAU患者外周血中Th17细胞和血清免疫球蛋白及补体水平存在差异,可能均参与了顽固性RAU的发生与发展,且Th17细胞与体液免疫之间的改变可能存在某种联系。

PURPOSE： To observe the levels of Th17 cells, immunoglobins and complements in peripheral blood of patients with refractory recurrent aphthous ulcer （refractory RAU） patients. METHODS： Totally, 30 patients with refractory RAU and 21 healthy controls were enrolled into this study. Flow cytometry was performed to determine the frequency of Th17 cells in the peripheral blood. Immune scatter turbidimetry was adopted to measure the levels of IgA, IgG, IgM, IgE, C3 and C4 in the peripheral blood. Meanwhile, the correlations between the frequency of Th17 cells and the levels of immunoglobins or complements were analyzed. SPSS17.0 software package was used to analyze the date. RESULTS： Compared with the control group, the percentage of Thl7 cells （CD3＊CDS-IL17＊） in all CD4CF cells was significantly increased in the refractory RAU group （P〈0.01）. Similarly, the levels of IgG, IgA, IgE in the refractory RAU group were significantly higher than those in the control group （P〈0.05）,but there was no significant difference in the levels of IgM, C3 and C4 between the refractory RAU group and control group. There was negative correlation between the frequency of Th17 cells and the level of IgA （r=-0.477, P〈0.01）. CONCLUSIONS： The levels of Thl7 cells, immunoglobins and complements in refractory RAU patients are significantly different from healthy human, which may play an important role in the pathogenesis of refractory RAU. There may be some relationship between Th17 cells and the change of humoral immunity in refractory RAU.