北京市密云区关节炎患者莱姆病血清学调查

Serological investigation in arthritis patients with Lyme disease in Miyun district, Beijing

目的了解北京市密云区关节炎患者伯氏疏螺旋体感染状况,为莱姆病防治工作提供科学依据。方法2016年在密云区采集关节炎患者血清标本,采用ELISA法对血清标本进行莱姆病抗体初筛检测,阳性血清标本采用免疫印迹法进行确证。结果共采集关节炎患者血清标本140份,莱姆病抗体阳性17份,总抗体阳性率为12.14%;莱姆病Ig M抗体和Ig G抗体阳性率分别为2.86%(4/140)和9.29%(13/140)。总抗体阳性率女性高于男性,50~岁年龄组总抗体阳性率最高,不同乡镇人群差异无统计学意义(χ2=10.385,P=0.320);临床以膝关节病变患者最多。结论密云区存在伯氏疏螺旋体感染导致的关节炎症状患者,因此临床医生在诊治关节炎患者时,应考虑莱姆病的鉴别诊断。

Objective To investigate the infection status in arthritis patients with Lyme disease in Miyun district,and to provide scientific basis for the prevention and treatment of Lyme disease.Methods Serum samples of patients with arthritis were collected,Lyme disease antibody screening in serum samples were detected by enzyme-linked immunosorbent assay,and positive serum samples were identified by western blot.Results A total of 140 serum samples were collected,17 samples were positive of Lyme disease antibody,the total positive rate was 12.14%.Four serum samples were positive for Lyme disease IgM antibody,the positive rate was 2.86%,13 serum samples were positive for Lyme disease IgG antibody,the positive rate was 9.29%.The total positive rate of antibody was higher in women than men,and the total positive rate of antibody was highest in 50-years old age group,there were no significant differences in total positive rate between different districts.Conclusion There were patients with arthritis caused by the Borrelia burgdorferi infection in Miyun district,so clinicians should consider Lyme disease in the diagnosis and treatment of arthritis patients.