转化生长因子、核心蛋白聚糖在慢性非细菌性前列腺炎大鼠模型前列腺组织中的检测及意义

Expressions of transforming growth factor-beta1 and DCN in rat models of chronic non-bacterial prostatitis and their clinical significance

目的 研究慢性非细菌性前列腺炎（chronic nonbacterial prostatitis,CNP） SD大鼠前列腺组织中转化生长因子（TGF-β1）和核心蛋白聚糖（DCN）的表达,并初步探讨前列腺组织纤维化的发病机制.方法 将60只6月龄SD大鼠随机分为对照组、30 d和45 d CNP模型组各20只.CNP模型组采用去势＋高剂量苯甲酸雌二醇肌注方法建立.采用双抗体夹心法检测SD大鼠前列腺组织中TGF-β1、DCN的含量.结果 ①45 d、30 d CNP模型组大鼠TGF-β1表达水平[（140.78 ±37.94）、（106.28 ±30.63） ng/L]均显著高于对照组[（13.42-±4.24）ng/L,P＜0.05];且45 d CNP模型组高于30 d CNP模型组（P＜0.05）;②45 d、30 d模型组DCN表达水平[（947.06±114.28）、（722.96±110.66） ng/L]显著高于对照组[（63.97±15.34） ng/L,P＜0.05];且45 d CNP模型组高于30 d CNP模型组（P＜0.05）;③TGF-β1、DCN在CNP大鼠模型前列腺组织中表达呈正相关.结论 TGF-β1、DCN可能参与CNP发病过程,在前列腺纤维化的形成中起着重要的作用.

Objective To investigate the expressions of transforming growth factor-beta1 and DCN in the prostatic tissue of rat models of chronic nonbacterial prostatitis （CNP）,and to explore the mechanisms of CNP and its fibrosis.Methods Sixty 6 month old SD rats were randomly allocated into three groups：normal control,30 d CNP model and 45 d CNP model,the models made by castration ＋high dose intramuscular injection of estradiol benzoate.The expressions of TGF-β1 and DCN in the prostatic tissue were measured using enzyme-linked immuno sorbent assay （ELISA）.Results ①TGF-β1 level was higher in the 45 d and 30 d CNP rat models [（140.78 ± 37.94） ng/L,（106.28 ± 30.63） ng/L] than controls [（13.42 ± 4.24） ng/L,P ＜ 0.05],even more significantly in the 45 d than in the 30 d group.②DCN level was higher in the 45 d and 30 d CNP rat models [（947.06 ±114.28） ng/L,（722.96±110.66） ng/L] than controls[（63.97-±15.34） ng/L,P＜0.05],even more significantly in the 45 d than in the 30 d group.③And the expression of TGF-β1 was positive correlated with that of DCN in the CNP rat models.Conclusions TGF-β1 and DCN may be involved in the pathogenesis of CNP,and have an important role of the formation of prostatic fibrosis.