3种有机磷农药间接竞争ELISA检测方法的建立

Development of Indirect Competitive Enzyme-linked Immunosorbent Assay for Detection of Three Organophosphorus Pesticides

[目的]建立能同时检测甲基对硫磷、杀螟硫磷和倍硫磷的间接竞争酶联免疫分析方法(ic-ELISA)。[方法]根据ic-ELISA检测的基本程序,利用能同时识别甲基对硫磷、杀螟硫磷和倍硫磷的宽谱特异性单克隆抗体,优化相关反应的理化参数,确定最佳反应条件。[结果]建立的ic-ELISA检测方法对甲基对硫磷、杀螟硫磷和倍硫磷的灵敏度(IC_(10)值)分别为8.81、11.73、13.25μg/L;检测范围分别为12.03μg/L^0.94 mg/L、15.84μg/L^1.07 mg/L和18.92μg/L^2.79 mg/L。[结论]建立的ic-ELISA方法可以满足甲基对硫磷、杀螟硫磷和倍硫磷在果蔬及谷物中最大残留限量的检测要求。

[Aims] An indirect competitive enzyme-linked immunosorbent assay（ic-ELISA） method for the detection of parathion-methyl, fenitrothion and fenthion was developed. [Methods] The reaction conditions of ic-ELISA were optimized based on a broad-specificity monoclonal antibody to parathion-methyl, fenitrothion and fenthion. [Results] The sensitivities（（IC_（10） value） of ic-ELISA for parathion-methyl, fenitrothion and fenthion were 8.81, 11.73 and 13.25 μg/L,respectively. The linear range for the three organophosphorus pesticides were 12.03 μg/L-0.94 mg/L, 15.84 μg/L-1.07 mg/L and 18.92 μg/L-2.79 mg/L, respectively. [Conclusions] The developed ic-ELISA method can meet the demand for detection of maximum residue amount of parathion-methyl, fenitrothion and fenthion in cereal, fruit and vegetable.