黔产乌头中的生物碱成分及其抗烟草花叶病毒(TMV)活性

Alkaloids of Aconitun carmichaeli Debx and against TMV activity

[目的]对黔产乌头(Aconitun carmichaeli Debx)根部甲醇提取物中碱性氯仿萃取的乌头生物碱总碱进行抗烟草花叶病毒(TMV)活性筛选。[方法]通过活性追踪从总碱中分离鉴定了14个C_(19-)型乌头生物碱成分,采用活体半叶枯斑法进行抗TMV活性筛选。[结果]发现其中achaconitine(11)、hemsleyanine C(12)、talatisamine(14)抗TMV侵染活性明显强于阳性对照药剂宁南霉素。[结论]在30 mg/L质量浓度下,该植物总碱抗TMV侵染抑制率为23.5%。Chaconitine(11)、hemsleyanine C(12)、talatisamine(14)可用于防治烟草花叶病毒的新型抗病毒剂。讨论乌头生物碱抗TMV侵染和增值的构效关系。

[Aims] Primary screening indicated that total alkaloids of Aconitun carmichaeli Debx had anti-tabacco mosaic virus（TMV） activity. [Methods] Guiding isolation of the total alkaloids resulted in separation of 14 C_（19-）-aconitine type alkaloids, the anti-TMV activity was screened by living half-leaf spotting method. [Results] Compounds achaconitine（11）, hemsleyanine C（12） and talatisamine（14） showed stronger activities against invasion of TMV disease than positive control ningnanmycin. [Conclusions] The inhibition rate of the total alkaloids against TMV was 23.5% at dosage of 30 mg/L. Compounds achaconitine（11）, hemsleyanine C（12） and talatisamine（14） as newly reported antiviral compounds could be used to control TMV diseases. The structure-activity relationship against on invasion and proliferation of TMV disease was also discussed.