抗支糖浆对哮喘大鼠BALF中IL-4、IL-13与IFN-γ含量的影响

Effect of Kangzhi Syrup on IL-4,IL-13 and IFN-γ in BALF of Asthma Rats

目的:观察抗支糖浆对哮喘大鼠支气管肺泡灌洗液(BALF)中IL-4、IL-13及IFN-γ含量的影响,探讨其治疗哮喘的作用机制。方法:48只雄性大鼠随机分为空白组、模型组、地塞米松组、抗支糖浆组四组,除空白组外,其余各组采用皮下注射10%OVA、2%氢氧化铝混合液致敏及2%OVA雾化激发的方法制作哮喘大鼠模型。各组分别用生理盐水、地塞米松及抗支糖浆进行灌胃,连续灌胃14天后取大鼠BALF,离心留取上清液。采用ELISA法测定BALF中IL-4、IL-13与IFN-γ的含量,并用HE染色法观察肺组织病理学改变。结果:与空白对照组比较,模型对照组BALF中IL-4、IL-13含量明显升高(P<0.05),IFN-γ含量明显降低(P<0.05);抗支糖浆组IL-4、IL-13含量跟模型组相比均明显降低(P<0.05),IFN-γ含量显著升高(P<0.05)。肺组织病理显示,抗支糖浆可以减少哮喘引起的炎细胞浸润、支气管粘膜充血、渗出物的生成。结论:抗支糖浆能有效治疗哮喘大鼠,其作用机制可能与调节哮喘大鼠IL-4、IL-13和IFN-γ水平有关。

Objective： To observe the effect of Kangzhi syrup on IL - 4, IL - 13 and IFN - γ in BALF of the asthma rats, and to investigate its mechanism for treating asthma. Methods ： 48 male rats were randomly divided into the blank group, the model group, Dexamethasone group and Kangzhi syrup group. The mixture injection of 10% OVA and 2% A1 （ OH）3, and 2% OVA inhalation were used to made asthma models in the rats. After 14 days of corresponding interven- tion, the levels of IL -4, IL - 13 and IFN -γ in BALF were detected wtih ELISA method, and pathological changes of the lung tissues were observed with HE - staining method. Results ： Compared to the blank group, the levels of IL - 4 and IL- 13 were increased significantly in BALF in the model group（P 〈0.05） ; and the level of IFN -γ was reduced significantly （P 〈0.05 ）. The levels of IL-4 and IL- 13 were apparently decreased and IFN-γwas significantly in- creased in Kangzhi syrup group than those in the model group （ P 〈 0.05 ）. The pathological observation showed that Kangzhi syrup can reduce the infiltration of inflammatory cells, the hyperemia and exudation of bronchial mucosa caused by asthma. Conclusion： Kangzhi syrup can treat asthma rats effectively, and the mechanism may be related to the regu- lation of IL -4, IL - 13, and IFN - γ levels.