7-二氟亚甲基-5,4'-二甲氧基染料木黄酮对大鼠压力性尿失禁模型肛提肌细胞增殖与凋亡的影响

Effect of 7-difluoromethy-5,4'-dimethoxygenistein on the levator ani cell proliferation of Wistar rats models of stress urinary incontinence

目的研究7-二氟亚甲基-5,4'-二甲氧基染料木黄酮(DFMG)对压力性尿失禁(SUI)Wistar大鼠模型肛提肌细胞增殖与凋亡的影响。方法采用模拟产伤及卵巢切除的方法建立SUI Wistar大鼠模型,检测漏尿点压力和腹部漏尿点压力,HE和Masson染色观察肛提肌组织。分离并培养大鼠肛提肌细胞,实验组分为正常对照组、SUI组、SUI+DFMG(2、4、8μmol·L-1)处理组。MTT和FCM分别检测细胞增殖和凋亡;western blot检测c-myc、Fas、caspase-8、bcl-2、bax、cyto-c和caspase-9。结果与对照组比较,SUI组肛提肌细胞增殖率下降,凋亡率升高,bcl-2表达下调,c-myc、Fas、caspase-8、bax、cyto-c、caspase-9表达上调;用DFMG处理SUI大鼠模型肛提肌细胞后,细胞增殖率升高,凋亡率下降,bcl-2表达升高,c-myc、Fas、caspase-8、bax、cyto-c、caspase-9表达减少。结论 DFMG可抑制SUI大鼠模型肛提肌细胞凋亡,促进细胞增殖,其机制可能与其上调bcl-2、下调c-myc、Fas、caspase-8、bax、cyto-c、caspase-9表达有关。

Objective To investigate the effects of 7 - difluoromethy - 5,4′ - dimethoxy - genistein （DFMG） on levator ani ceil proliferation and apoptosis of Wistar rats models of stress urinary incontinence （SUI）. Methods The models of SUI of Wistar rats were made by imitating delivery damage and ovary removing surgery, and the effect of models were detected using leak point pressure （LPP）, abdominal leak point pressure （ALPP） and Histopathological examination of HE staining and Masson staining. The cells of levator ani of Wistar rats were isolated and cultured. The experimental group was divided into control group, SUI group, and SUI ＋ DFMG （2,4,8 μmol · L^-1） groups. MTT and FCM were used to detect cell proliferation and apoptosis, respectively. Western blot was used to determine the protein expression levels of c - myc、 Fas 、 caspase - 8 、 bcl - 2、 bax、 cyto - c and caspase - 9. Results Com- pared with the control group, the cell proliferation rate was decreased and the apoptotic rate was increased in the SUI group, concomi- tant with downregulation of bel - 2 and upregulation of c - mye, Fas, easpase - 8, bax, cyto - c and caspase - 9. DFMG could signifi- cantly improve the proliferation rate of levator ani cells and inhibit the apoptotic rate of levator ani cells, accompanying the increase of bcl - 2 and the decrease of e - myc, Fas, caspase - 8, bax, cyto - c and caspase - 9. Conclusion DFMG could inhibit levator ani cell apoptosis of SUI Wistar rats, promote cell proliferation, the underlying mechanism may be associated with upregulation of bcl - 2 and downregulation of c - myc, Fas, caspase - 8, bax, cyto - c and caspase - 9.