番茄颈腐根腐病病原鉴定及2种接种方法的评价

Pathogen Identification and Two Inoculation Method of Fusarium Crown and Root Rot

为了确定引起河北省丰南区发现的一种植株青枯、维管束褐变的番茄病害的病原菌,进行了病原菌分离培养纯化、致病性测定、田间接种后再分离、菌落和形态学观察结合分子鉴定,确定该病害为尖孢镰刀菌番茄颈腐根腐病专化型引起的番茄颈腐根腐病。确定病原菌为尖孢镰刀菌番茄颈腐根腐病专化型。对茎基接种和浸根接种进行了评价,结果表明,茎基接种发病率和死亡率均高于浸根接种。为接种完成该病害的抗病育种和防治药剂筛选的研究提供了理论依据。

In order to identify the pathogenic fungi which causing tomato bacterial wilt in Fengnan District of Hebei Province. This study proved the disease as Bipolaris sorokiniana by pathogen isolation,pathogenicity test,morphological observation,and r DNA-ITS gene sequence analyses was caused by Fusarium oxysporum f. sp. radicis-lycopersici（ Forl）. The result showed that the mortality and morbidity of stem based inoculation were higher than root soaking inoculation. So,the disease with stem based inoculation developed faster. The study provided theoretical basis for the breeding of disease resistance and the screening of the fungicides.