大黄素抑制宫颈癌Hela细胞增殖与诱导凋亡的研究

Effects of Emodin on Proliferation and Apoptosis of Cervical Cancer Hela Cells Lines

研究大黄素对宫颈癌Hela细胞的增殖与凋亡的影响。通过MTT法检测Hela细胞的存活率,Annexin V-FITC/PI双染法及流式细胞术检测Hela细胞凋亡,蛋白质免疫印记法检测p-JNK、JNK、Bcl-2、pro-caspase-9、cleaved-caspase-3蛋白的表达。大黄素对Hela细胞的增殖抑制效果呈浓度依赖性。Annexin V-FITC/PI双染和流式细胞术结果表明大黄素能够诱导Hela细胞凋亡。蛋白质免疫印记法表明,随着药物处理时间的增加,p-JNK、cleaved-caspase-3的蛋白质表达量逐渐增加,Bcl-2、procaspase-9的蛋白表达量逐渐减少。在实验条件下,大黄素可以抑制Hela细胞的增殖,诱导细胞凋亡。

To investigate the inhibitory effect of emodin on the proliferation of human cervical cancer,the viability of Hela cells was determined by MTF assay. Cells apoptosis were observed by Annexin V-FITC/PI staining and FCM. The expression levels of p-JNK, JNK,Bcl-2,pro-caspase-9 and cleaved-caspase-3 were determined by Western blotting. Emodin could inhibit the proliferation of Hela cells and in a dose-dependent manner. Annexin V-FITC/PI double staining and FCM results showed that emodin induced Hela cell apoptosis. In addition,it was suggested that western blot results showed that the expressions of p-JNK, cleaved-caspase-3 kept increasing, while Bcl-2, pro-caspase-9 decreased. Under this experimental condition, emodin could inhibit the proliferation of Hela cells and induce apoptosis by up-regulation JNK signaling pathway.