骨形成蛋白2在脑多形性胶质母细胞瘤中的表达及临床意义

Analysis of the Clinical Significance of Bone Morphogenetic Protein 2 in Glioblastoma Multiforme

目的分析人多形性胶质母细胞瘤(GBM)中骨形成蛋白2(BMP2)表达的预后意义和可能的相关信号机制。方法利用癌症基因组图谱(TCGA)数据库获取GBM标本的RNASeq V2数据,从人类肿瘤相关基因表达汇编(GEO)数据库下载GBM样本数据集GSE7696的表达谱数据。利用单因素Kaplan-Meier和多因素COX逐步回归法确定BMP2表达与GBM患者预后的关系。利用基因集富集分析方法预测BMP2相关的基因通路。结果低表达BMP2是GBM术后患者预后不良的独立因素。BMP2低表达富集了干扰素α反应的相关通路。结论 GBM组织中BMP2低表达预示患者预后不良,其作用机制可能是干扰素α反应基因异常。

Objective To investigate the prognostic significance of bone morphogenetic protein 2 （BMP2） expression in glioblastoma muhiforme （GBM） and related pathways. Methods RNASeqV2 data and GSE7696 series matrix data were downloaded from The Cancer Genome Atlas （TCGA） and The Gene Expression Omnibus （GEO） database, respectively. The correlation between BMP2 expression and prognosis was evaluated using Kaplan-Meier and multivariate COX analysis. Gene set enrichment analysis （GSEA） was used to predict the functional gene sets and/or pathways modulated by BMP2. Results Decreased expression of BMP2 is an independent predictor of poor prognosis. Furthermore, genes that are upregulated in response to interferon- α proteins were enriched in samples with low BMP2 expression. Conclusion Low expression of BMP2 indicates poor prognosis in GBM patients. Genes that are upregulated in response to interfer- on- α proteins may also be implicated in prognosis.