长链非编码RNA HOTAIR对外阴鳞状细胞癌A431细胞生物学行为的影响

Effects of Long Non-coding RNA HOTAIR on the Biological Behaviors of Vulvar Squamous Cell Carcinoma A431 Cells

目的探讨长链非编码RNA(lnc RNA)HOTAIR对外阴鳞状细胞癌A431细胞生物学行为的影响。方法采用实时荧光定量PCR检测外阴鳞状细胞癌A431细胞与正常表皮HaCaT细胞中HOTAIR的表达水平,转染HOTAIR si RNA至A431细胞后采用CCK-8法检测各组细胞活性,流式细胞术检测细胞凋亡,Transwell小室迁移实验检测细胞迁移情况,Transwell小室侵袭实验检测细胞侵袭情况。结果 HOTAIR在A431细胞中的表达高于正常表皮HaCaT细胞;下调A431细胞中HOTAIR表达后,细胞增殖、迁移及侵袭能力均降低(P均<0.05),而凋亡率增加(P<0.05)。结论 lnc RNA HOTAIR可能在调控外阴鳞状细胞癌细胞的增殖、凋亡、迁移及侵袭行为中起重要作用。

Objective To investigate the effects of long non-coding RNA （lncRNA） HOTAIR on the biological behavior of the vulvar squamous cell carcinoma A431 ceils. Methods The expression level of HOTAIR in vulvar squamous cell carcinoma A431 ceils and normal epidermis HaCaT cells were detected by real-time PCR. A431 cells were transfected with HOTAIR siRNA, then the proliferation, apoptosis, migration activities, and invasion activities were measured by CCK-8 assay,flow cytometry assay, transwell chamber migration test, and transwell chamber invasion test, respectively. Results After down-regulating the level of HOTAIR in A431 cells, the cell proliferation, migration, and invasion abilities were decreased （P 〈 0.05）, while the cell apoptosis rate was increased （P 〈 0.05）. Conclusion lncRNA HOTAIR may play an important role in the proliferation, apoptosis, migration, and invasion in vulvar squamous cell carcinoma.