中药复方脊髓康对小胶质细胞吞噬神经元碎片作用的影响

Effect of Chinese herbal compound "Jisuikang" on engulfment of neuron debris by microglia

目的观察中药复方脊髓康(JSK)对小胶质细胞吞噬神经元碎片的影响。方法制备JSK含药血清,分为低、中、高剂量组,另设空白血清组、脂多糖(LPS)+空白血清组;慢病毒转染绿色荧光蛋白基因(GFP)标记小胶质细胞;分离、鉴定原代神经元,建立损伤神经元模型,并将损伤神经元与转染后小胶质细胞混合培养。荧光显微镜下观察含药血清干预后小胶质细胞吞噬神经元碎片的情况。结果 JSK中、高剂量组小胶质细胞吞噬百分率及吞噬指数与空白组对比差异具有统计学意义(P<0.001);与和脂多糖+空白血清组比较,JSK高剂量组在吞噬百分率方面无差异,而在吞噬指数方面具有明显优势(P<0.05)。结论中药复方JSK能促进小胶质细胞吞噬神经元碎片,为神经元生长提供良好的生存环境。

Objective To investigate the effect of Chinese herbal compound ＂Jisuikang＂ on the phagocyto-sis of neuronal debris by microglial cells. Methods To prepare serum containing drugs of JSK and divide them intothe low,middle and high dose groups,the blank serum group and LPS ＋ blank serum group. BV2 was labeled bylentiviral vectors containing the green fluorescent protein gene（GFP）. To establish the damage neuron model andmix injured neurons with the transfected microglia. To observe the situation of microglia which was affected by serumcontaining drugs devour the neuronal debris. Results The middle and high dose of JSK showed greater phagocyticpercentage and phagocytic index than those of the control group（P 0.001）. In comparison of LPS ＋ blank serumgroup,no significant difference was found in the middle and high dose of JSK. However,to the phagocytic index,which was better than that of LPS ＋ blank serum group（P 0.05）. Conclusion JSK may enhance the engulfmentof neuron debris by BV2,which could provide a better living environment for the growth of neurons.