柚皮素对肠缺血再灌注损伤的保护作用

Protective effect of Naringenin in intestine ischemia reperfusion injury

目的探讨柚皮素(naringenin,NAR)对肠缺血再灌注损伤的作用及分子机制。方法 30只SD大鼠随机分成假手术组(sham)、肠缺血再灌注损伤组(IRI)和柚皮素预处理组(NAR)(n=10)。检测各组血清肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6),白细胞介素1((IL-1(),PI3K,AKT,Bax和BCL-2表达水平以及肠组织病理形态。结果与Sham组相比,IRI组IL-6、IL-1β、TNF-α、PI3K、AKT和Bax表达水平以及肠道病理改变明显增加,而BCL-2表达明显降低。与IRI组相比,NAR组IL-6、IL-1β、TNF-α、PI3K、AKT和肠道病理改变明显减少,而BCL-2表达明显增加,差异有统计学意义(P<0.05)。结论NAR可通过减轻肠缺血再灌注损伤,机制与抑制PI3K/AKT介导的炎症和凋亡相关。

Objective To explore the effect and molecular mechanism of Naringenin（NAR）on intestine ischemia reperfusion injury in rats.Methods 30 SD rats were randomly divided into sham group（Sham）,intestinal ischemia reperfusion injury group（IRI）and NAR group（NAR）（n=10）.The expression level of tumor meerosis factor-α（TNF-α）,interleukin-1（IL-1）,interleukin-6（IL-6）,PI3 K,AKT,Bax and BCL-2were examined.The pathological morphology of intestinal tissue was also measured by HE.Results Compared with the Sham group,the pathological change of intestinal and the expression of IL-6,IL-1,TNF-,PI3 K,AKT and Bax in IRI group were significantly increased.But the expression of BCL-2in IRI group was significantly decreased.Compared with the IRI group,the pathological change of intestinal and the expression of IL-6,IL-1,TNF-,PI3 K,AKT and Bax in NAR group were significantly decreased.But the expression of BCL-2in NAR group was significantly increased.Conclusion NAR can decrease intestine ischemia-reperfusion injury,the mechanism of which is associated with suppressing PI3K/AKT mediating inflammation and apoptosis.