摘要
目的探讨长链非编码RNA(long non-coding RNAs,lncRNAs)MALAT1在冠状动脉粥样硬化性心脏病(冠心病)患者外周血清中的表达水平及其临床意义。方法采用实时定量聚合酶链反应检测122例冠心病患者(冠心病组)和48例冠状动脉造影检查阴性人群(对照组)血清lncRNA MALAT1表达水平;将冠心病组患者分为未接受他汀类药物治疗亚组和接受他汀类药物治疗亚组,分析冠心病患者血清lncRNA MALAT1表达水平差异。采用单因素及多因素分析影响冠心病患者临床预后的危险因素。结果与对照组相比,冠心病组血清lncRNA MALAT1的表达水平明显升高,差异具有统计学意义(P<0.05);冠心病未接受他汀类药物治疗亚组血清lncRNA MALAT1表达水平高于接受他汀类药物治疗亚组,差异具有统计学意义(P<0.05)。此外,单因素分析发现患者的年龄、吸烟、低密度脂蛋白胆固醇(LDL-C)浓度、高密度脂蛋白胆固醇(HDL-C)浓度、糖尿病、原发性高血压(高血压)及血清MALAT1的表达是冠心病的危险因素(P<0.05);进一步多因素分析发现高血压、低密度脂蛋白胆固醇浓度及血清MALAT1表达水平是冠心病的独立危险因素(P<0.05)。结论冠心病患者外周血清中lncRNA MALAT1表达水平升高;他汀类药物可降低冠心病患者血清lncRNAMALAT1表达水平。lncRNA MALAT1可能是潜在的冠心病预后预测的血清标志物。
Objectives To investigate the expression and clinical significance of long non-coding RNAs （lncRNAs） MALAT1 in patients with coronary atherosclerotic heart disease（CAD）. Methods Serum lncRNA MALAT1 expres-sion levels were measured in 122 patients with CAD（CAD group）and 48 cases with negative coronary angiography re-sults （control group） by quantitative real time polymerase chain reaction （QRT-PCR）. Patients in CAD group were further divided into receiving statin therapy subgroup and not receiving statin therapy subgroup. Serum lncRNA MALAT1 expression levels were compared between the two subgroups. Risk factors for clinical outcomes of patients with CAD were analyzed by univariate factor and multiple factor analysis. Results Compared with control group ,serum ln-cRNA MALAT1 expression levels were significantly increased in CAD group（P〈0.05）. Serum lncRNA MALAT1 ex-pression levels in not receiving statin therapy subgroup was significantly higher than that in receiving statin therapy sub-group（P〈0.05）. In addition,univariate analysis found that the patient＆#39;s age,smoking,low density lipoprotein choles-terol（LDL-C）concentration,high density lipoprotein cholesterol（HDL-C）concentration,diabetes,hypertension and serum lncRNA MALAT1expression level were risk factors for CAD（P〈0.05）;further multivariate analysis found that hypertension,LDL-C concentration and serum lncRNA MALAT1 expression level were independent risk factors for CAD （P 〈 0.05）. Conclusions Serum lncRNA MALAT1 expression level in patients with CAD significantly de-crease,which may be as a potential marker for diagnosis and prognosis of CAD.
出处
《岭南心血管病杂志》
2017年第5期506-510,共5页
South China Journal of Cardiovascular Diseases
