摘要
目的利用RNA干扰技术探讨p16^(INK4a)对人毛乳头细胞(dermal papilla cell,DPC)的促进作用。方法将针对p16^(INK4a)基因设计的RNA腺病毒转染高传代DPC,观察细胞生长活性,电镜下观察细胞形态,western blot检测衰老相关蛋白β-半乳糖苷酶、pRb的表达。结果转染后细胞倍增时间缩短,细胞核分裂增多,高传代DPC中β-半乳糖苷酶、pRb蛋白较低传代DPC均上调,转染后两种蛋白的表达呈下降趋势。结论高传代DPC非凝集性生长与细胞过早性衰老有关,DPC的p16^(INK4a)基因沉默后可延缓DPC衰老,促进DPC增殖。
Objective Study the effect of p16INK4a on dermal papilla cells(DPCs)by RNA interference technique.Methods High passage DPCs in vitro were transfected by RNA adenovirus designed according to p16INK4a gene sequence to observe the cell growth activity, and the cell morphology was observed by electron microscope.The expression of senescence related protein was detected by western blot.Results The cells doubling time of transfected group was shortened and the cell division was increased compared with normal group.The expression of pRb and β-galactosidase protein in high passage DPCs was up-regulated compared with low passage, but decreased after transfection.Conclusion Non-aggregative growth of DPCs is associated with premature aging.Knockdown of p16INK4a inDPCs can delay DPCs senescence and promote DPCs proliferation.
出处
《中国皮肤性病学杂志》
CSCD
北大核心
2017年第11期1183-1187,共5页
The Chinese Journal of Dermatovenereology