摘要
为更深入的研究和开发利用贵州无籽刺梨和刺梨资源,本研究采用盐酸水解法,以正亮氨酸为内标物,异硫氰酸苯酯(PITC)为柱前衍生剂,用高效液相色谱测定无籽刺梨与野生刺梨的氨基酸的含量,对其进行组成分析和营养评价。结果表明,三种果实样品均含有16种氨基酸,成熟无籽刺梨与刺梨比较,无籽刺梨的总氨基酸、必需氨基酸、儿童必需氨基酸、味觉氨基酸、药效氨基酸、支链氨基酸略高;氨基酸组成分析中,除了Cys+Met外,其它均符合WHO/FAO氨基酸模式谱。
To provide scientific basis for further research and development for Rosa sterilis and Rosa roxburghii in Guizhou,this paper adopt hydrochloric acid hydrolysis method, using norleucine as the internal standard, then derivatized with phenylisothiocyanate（ PITC） and determined by HPLC,analysis the amino acids composition and nutritional evaluation at last.Results showed that three samples contained 16 kinds of amino acids. Compared with Rosa roxburghii,total amino acid,essential amino acids,children＇s essential amino acids,taste amino acids,pharmacodynamics amino acids and branched chain amino acid in the fruit of Rosa sterilis were higher. Analysis of amino acid composition,in addition to Cys ＋ Met,others are in accordance with WHO/FAO amino acid pattern spectrum.
出处
《山东化工》
CAS
2017年第18期76-79,共4页
Shandong Chemical Industry
基金
州省农业科技攻关项目(黔科合NY[2015]3022-1号)
教育部长江学者和创新团队发展计划(IRT1227)
贵州省林业厅项目(黔林科合[2016]09)
贵州师范大学博士科研基金
关键词
高效液相色谱法
无籽刺梨
刺梨
氨基酸
营养评价
high performance liquid chromatography （HPLC）
Rosa sterilis
Rosa roxburghii
amino acids
nutritional evaluation
