摘要
通过添加外源过氧化氢来探究其对植物生长素信号的影响.以GUS基因作为报告基因,研究拟南芥中各生长素相关蛋白(生长素含量标志蛋白DR5、输入蛋白AUX1、输出蛋白PIN1和PIN2)对过氧化氢的响应.结果表明过氧化氢处理后拟南芥呈植株变小、根变短和不定根数目增多的表型;与对照相比,GUS染色后各生长素相关蛋白的表达下降,表明过氧化氢对植物生长素的合成与运输起一定的抑制作用.在过氧化氢处理的基础上,添加抗氧化物质后发现植株表型有明显恢复,GUS染色也表明相关蛋白的表达量均增加;另外,0.1 nmol/L的外源IAA能恢复过氧化氢处理后拟南芥的表型和生长素相关蛋白的表达.综上所述,过氧化氢主要通过抑制植物体内的生长素信号来调控植物的生长发育.
The effect of exogenous hydrogen peroxide on the auxin signal is studied. The GUS was used as the reporter gene to investigate the responses of auxin signaling proteins,including DR5(a maker of auxin level),AUX1(auxin input protein),PIN1 and PIN2(auxin output proteins) to hydrogen peroxide. The results showed that Arabidopsis thalianas displayed smaller size,shorter root and increased adventitious root number after hydrogen peroxide treatment. The GUS staining results showed that the expression of auxin-related proteins decreased,and suggested that hydrogen peroxide can inhibit the auxin synthesis and transport. The effects of hydrogen peroxide on the phenotype and the expression of auxin-related proteins in A. thaliana can be restored by some antioxidants,as well as 0.1 nmol/L IAA. The results suggest that the hydrogen peroxide can regulate the plant growth and development by inhibiting the auxin signal.
出处
《华南师范大学学报（自然科学版）》
CAS
北大核心
2017年第5期69-77,共9页
Journal of South China Normal University(Natural Science Edition)
基金
广东省自然科学基金项目(2014A030313443)
2011年广东省教学团队
2013国家级精品课程资源共享课程建设项目(植物生理学)
关键词
拟南芥
生长素信号
过氧化氢
生长发育
Arabidopsis thaliana
auxin signal
hydrogen peroxide
growth and development
