GC-MS法测定分析吴茱萸挥发油的有效化学成分

Analysis of Volatile Oil of Euodia Rutaecarpa by GC-MS Method

尝试快速分析吴茱萸果实中的挥发油组分,为后续的吴茱萸功效-毒性物质基础研究提供依据.通过水蒸气蒸馏法提取挥发油,运用气相色谱—质谱(GC-MS)联用技术测定了吴茱萸挥发油中所含的化学成分和其相对含量.结果表明:一共含有48种化合物,占吴茱萸挥发油含量的93.99%,其中相对含量较高的成分有(Z)-罗勒烯(60.57%)、(E)-罗勒烯(9.11%)、月桂烯(5.94%)、石竹烯(1.78%)、β-榄香烯(2.04%).吴茱萸挥发油中的化学成分十分丰富,使用GC-MS方法可以快速高效地鉴别它的有效挥发油成分.

It is to analyze the volatile oil of euodia rutaecarpa( ER),and provide the experimental data for the subsequent efficacy and toxicity research of ER. The volatile oils were extracted from ER by steam distillation.The component analysis and the relative content of volatile oil were carried out by GC-MS under the optimum conditions. A total of 48 compounds were identified in this experiment,accounted for 93. 99% of the total volatile oil.Some relatively higher content compounds consist of( Z)-Ocimene( 60. 57%),( E)-Ocimene( 9. 11%),Myrcene( 5. 94%),Caryophyllene( 1. 78%),and β-Elemen( 2.04%).The volatile oil of ER contains abundant chemical composition.GC-MS method is a fast,sensitive and reliable tool for the component analysis of volatile oil.