基于微流控芯片的细菌趋化性检测研究进展

Advance in Bacteria Chemotaxis on Microfluidic Devices

细菌趋化性是指有运动能力的细菌对环境化学物质梯度产生响应,趋向某些化学诱导剂或避开某些化学驱避剂的移动行为,是微生物适应环境变化而生存的一种基本属性。研究细菌趋化性对于利用细菌治理环境、控制病原菌侵染机体以及开发微生物工业项目等方面都具有重要意义。微流控芯片可以实现对细菌趋化性的定性与定量检测,与传统的检测方法相比,可以更好地对细菌的微环境进行控制,有较高的灵敏度。近年来,基于微流控技术检测细菌趋化性研究得到了飞速发展。本文从微流控芯片的结构、工作方式及主要应用3个方面对近年出现的微流控趋化性检测装置进行了介绍和评述。

Chemotaxis is the response ability of motile cells to chemicals gradients in environment and the migration toward higher concentration of chemoattractant or lower concentration of repellent. This mechanism is a basic nature of microorganisms to adapt to the environmental changes. The research of microbial chemotaxis is of great significance in utilizing bacteria to solve environment problems,control the pathogen infection,and develop microbial industrial projects. Microfluidic devices can realize qualitatively and quantitatively detect of bacterial chemotaxis. In comparison with traditional detect methods,microfluidic assay has an accurate control over bacterial microenvironment,with a higher sensitivity. In the past few years,bacterial chemotaxis study based on microfluidic assay was developed rapidly. In this paper,the microfluidic chemotaxis detectors that appeared in recent years were introduced from the aspect of chip structure,working principle and their applications. Finally,we provided insights into the challenges of bacterial chemotaxis and provided future perspectives.