灰葡萄孢毒素对产后蒜薹的致病性及其毒素基因的检测

Pathogenicity to Garlic Scape and Gene Detection of Toxin from Botrytis cinerea

为明确灰葡萄孢毒素对产后蒜薹的致病性及其毒素基因的检测方法,本研究运用蒜薹灰霉病强致病菌株灰葡萄孢霉BC-3,通过蒜薹悬滴接种法、系统侵染法和毒素浸渍法,测定灰葡萄孢毒素对蒜薹薹条和薹苞组织损伤及细胞叶绿素降解的活性。结果表明,灰葡萄孢毒素是导致产后蒜薹灰霉病的重要因素,可造成蒜薹组织细胞损伤、叶绿素降解,并可由输导组织传导造成蒜薹组织系统侵害。同时建立了灰葡萄孢毒素Bc BOT2基因特异性检测方法,可用于其引起的蒜薹灰霉病早期检测。

To make clear the pathogenicity of toxin from Botrytis cinerea to garlic scape and the detection method to toxin gene,the Botrytis cinerea strain BC-3 with high pathogenicity was selected as target. The bioassay of mycotoxin to tissue damage and cell chlorophyll degradation activity were tested by garlic scape droplet inoculation,systemic infection and toxin dipping. The results showed that the toxin of Botrytis cinerea was the important pathogenic factor leading to garlic gray mold. The toxin could lead to tissue injury,chlorophyll degradation and garlic conducting tissue damage. At the same time,the specific detection method to toxin gene Bc BOT2 was established. The method could be used to the early detection of garlic gray mold caused by Botrytis cinerea.