HPV检测在宫颈腺癌不同亚型中的临床分析

Clinical analysis of HPV detection in various subtypes of cervical adenocarcinoma

目的探讨原发性宫颈腺癌的不同亚型中HPV感染情况及临床意义。方法综合2003版WHO及文献中的形态分类标准对116例宫颈腺癌标本进行组织亚型分类。采用SPF10 PCR技术进行DNA扩增,检测宫颈腺癌的HPV DNA。对所有石蜡切片进行病理阅片和诊断,分析子宫颈腺癌标本不同亚型中的HPV感染情况。结果 116例宫颈腺癌中,HPV总阳性率为76.7%。共检出HPV感染者89例,均为高危型别感染,主要是HPV16和18感染。采用<50岁以及≥50岁作为年龄分组,不同年龄组高危HPV感染的差异有统计学意义(P=0.018);不同腺癌病理组织亚型间,高危HPV感染的差异具有统计学意义(P=0.011)。结论 HPV16和HPV18为宫颈腺癌各个亚型的主要感染型别,HPV检测是目前筛查和预防宫颈腺癌的关键之一。

Objective To study HPV infection and its clinical significance in various subtypes of cervical adenocarcinoma.Methods 116 cases of cervical adenocarcinoma were classified according to World Health Organization（WHO） and other associated literatures. DNA was amplified by SPF10-PCR method. DNA genotyping used Li PA method. All biopsy specimens had pathologic diagnosis. Results 116 adenocarcinoma cases were included in this study. HPV positive rate was 76.7%. There were 89 cases of the high-risk HPV infection. HPV 16 and HPV 18 were two most prevalent HPV types in cervical adenocarcinoma. When the patients younger than 50 years old were compared with the patients equal or older than 50 years old as two age groups,the high-risk HPV positive rate showed significantly statistical difference（P=0.018） between the two age groups. The high-risk HPV positive rate presented significantly statistical difference（P =0.011） in various subtypes of cervical adenocarcinoma. Conclusion HPV16 and HPV18 are two most prevalent HPV types in various subtypes of cervical adenocarcinoma. HPV detection is one of the keys to screening and preventing cervical adenocarcinoma.