中国南北方山楂叶UPLC指纹图谱比较分析

COMPARATIVE ANALYSIS OF UPLC FINGERPRINTS OF HAWTHORN LEAVES IN SOUTH AND NORTH CHINA

目的:通过建立基于主成分分析(PCA)的山楂叶超高效液相色谱(UPLC)指纹图谱对我国南北方山楂叶进行比较分析。方法:采用UPLC法对我国北方(10批)、南方(6批)共16批山楂叶进行分析,建立基于PCA的山楂叶UPLC指纹图谱。结果:通过主成分分析筛选出9批样品、6批样品,分别建立北方和南方山楂叶UPLC指纹图谱。北方山楂叶指纹图谱得到16个共有峰,相似度为0.808-0.972;南方山楂叶指纹图谱得到23个共有峰,相似度为0.923-0.979。结论:本研究构建的基于PCA的山楂叶UPLC指纹图谱能客观评价我国南北方山楂叶药材的差异,可为山楂叶的临床应用提供参考。

Objective：To comparatively analyze the hawthorn leaves in south and north China by establishing HPLC fingerprints based on principal component analysis（PCA）. Methods： HPLC was used to analyze 16 batches of hawthorn leavesfrom south（6 batches） and north（10 batches） China, and established HPLC fingerprints based on PCA. Results： 9 batches from north China and 6 batches from south China were selected to establish the UPLC fingerprints of hawthorn leaves based on PCA. The HPLC fingerprints of hawthorn leaves from north China had 16 common peaks, and the similarity was 0.808-0.972; the HPLC fingerprints of hawthorn leaves from south China had 23 common peaks, and the similarity was 0.923-0.979. Conclusions： The HPLC fingerprints of hawthorn leaves based on PCA in this study can objectively evaluate the differences of hawthorn leaves in south and north China, so it can provide references for clinical application of hawthorn leaves.