直接法与公式法测定小而密低密度脂蛋白的比较研究

Comparative Study on the Determination of Small Dense Low Density Lipoprotein by Direct and Formula Methods

目的探讨直接法与公式法测定小而密低密度脂蛋白(sdLDL)的差异。方法对2016年7月—2017年6月门诊收集的3 346份血标本进行血脂检测,包括总胆固醇(TC)、低密度脂蛋白胆固醇(HDL-C)、三酰甘油(TG)等血脂全套。再根据高脂血症类型,对比分析直接法与公式法测定的sdLDL结果。结果各型高脂血症及正常对照组中直接法与公式法测定的sdLDL含量均具有较好的相关性,其中正常对照组及低HDL-C血症组相关性最好(r值分别为0.830和0.831);混合型高脂血症组及低HDL-C血症组直接法与公式法差异无统计学意义。结论公式法估算sdLDL可应用于健康对照人群,但对于高脂血症病人应采用直接法测定sdLDL含量,才能为临床提供可靠的检验数据,指导筛查和治疗。

Objective To study the difference between direct and formula method for the determination of small dense low density lipoprotein（sdLDL）.Methods Three thousands,three hundreds and forty-six blood samples were collected from July to November in our hospital,and tested for serum lipids,including total cholesterol（TC）,low density lipoprotein（HDL）and triglyceride（TG）.And then according to the type of hyperlipidemia,the sdLDL results were determined by direct and formula methods.Results There was a correlation between the content of sdLDL measured by direct method and formula method in all types of hyperlipidemia and normal control group.The correlation between the normal control group and the low HDL-C group was the best（correlation coefficient was 0.830 and 0.831,reppectively）.There was no significant difference between the direct method and the formula method in the mixed hyperlipidemia group and the low HDL-C group.Conclusion The sdLDL can be applied to screening healthy population,but it is necessary for patients with hyperlipidemia to determine the content of sdLDL by direct method to provide reliable test data for clinical diagnosis and treatment.