泽泻提取物对STZ糖尿病大鼠的干预作用

Intervention effects of the extract of Alismatis Rhizoma on streptozotocin-induced type 2 diabetic rats

为评价泽泻乙酸乙酯部位对2型糖尿病的降血糖作用及其机制,采用高脂高糖饲料喂养大鼠4周后再腹腔注射小剂量链尿佐菌素(STZ)的方法,建立2型糖尿病大鼠模型。将糖尿病大鼠随机分为模型组、泽泻乙酸乙酯部位组(AREEA,20,50,100 mg/kg)、二甲双胍组(100 mg/kg)。连续给药4周后进行葡萄糖耐量和胰岛素耐量实验。于末次给药后禁食12 h,采血测定大鼠空腹血糖(GLU)、糖化血红蛋白(HbA1c)、胰岛素(Ins)以及胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)含量,脂肪组织中胰岛素信号通路IRS-1和Akt的磷酸化水平及胰脏的病理变化。结果显示,给药组大鼠的空腹血糖有明显降低(P<0.05);100 mg/kg组显著降低血清GLU、HbA1c、TC、TG、MDA、TNF-α、IL-6(P<0.05);明显升高SOD、GSH-Px(P<0.05)。这些结果提示泽泻乙酸乙酯部位可有效调节体内糖、脂代谢,对2型糖尿病有一定治疗作用。

To investigate the hypoglycemic effects of ethyl acetate extract of Alismatis Rhizoma（AREEA）on type 2 diabetes mellitus,the model of type 2 diabetic rats was induced by high-fat diet feeding for 4 weeks and then intraperitoneal injection of a low dose of streptozotoin（STZ）.Rats without the above-mentioned treatment were selected as the normal control group.The diabetic rats were randomly divided into 5 groups：the model control group,low doses（20 mg/kg）,medium doses（50 mg/kg）,high doses（100 mg/kg）of AREEA groups and positive control-metformin group（100 mg/kg）.After four weeks,oral glucose tolerance test（OGTT）and insulin tolerance test（ITT）were performed,respectively.12 hours after the last administration,the levels of serum glucose,glycated hemoglobin（HbA1c）,insulin（Ins）,total cholesterol（TC）,triglyceride（TG）,high density lipoprotein（HDL-C）,low density lipoprotein（LDL-C）,superoxide dismutase（SOD）,malondialdehyde（MDA）,glutathione（GSH-Px）,tumor necrosis factor-α（TNF-α）,interleukin-6（IL-6）,insulin-mediated tyrosine of IRS-1 and Akt phosphorylation in adipose tissue were determined.In addition,the pathological changes of pancreas were examined.After administration for 4 weeks,all doses of AREEA significantly reduced the fasting blood glucose of type 2 diabetic rats（P〈0.05）.In the high doses group of AREEA,the levels of GLU,HbA1c,TC,TG,MDA,TNF-α and IL-6 in serum were decreased significantly,and the levels of SOD and GSH-Px were increased（P〈0.05）.These results suggest that AREEA has the therapeutic effect on type 2 diabetes-related symptoms by metabolic regulation of glucose and lipids.