鱼芩解毒丸体外抗HIV-1活性探讨

Anti-HIV-1 Activities of Yu Qin Jie Du Pill in vitro

目的:探讨医院制剂鱼芩解毒丸的体外抗HIV-1病毒活性。方法:采用MTT比色法检测鱼芩解毒丸的细胞毒性;采用细胞病变法检测鱼芩解毒丸对HIV-1急性感染的抑制活性;采用HIV-1Ⅲ_B慢性感染细胞的p24抗原表达抑制实验检测鱼芩解毒丸的抗H1V-1活性。结果:鱼芩解毒丸抑制HIV-1Ⅲ_B诱导C8166细胞形成合胞体的EC_(50)为0.035~0.070 mg·mL^(-1),治疗指数为42.79~57.03 mg·mL^(-1);鱼芩解毒丸抑制HIV-1Ⅲ_B感染C8166的p24抗原表达的EC_(50)为0.070~0.090 mg·mL^(-1),治疗指数22.82~28.51 mg·mL^(-1)。结论:鱼芩解毒丸具有一定的抗HIV-1活性。

Objective： To assess the anti-HIV-1 activity of a hospital made pill named yu qin jie du pill in vitro. Methods： To detect cytotoxicity of the yu qin jie du pill by MTT staining method; to detect inhibitory activity of yu qin jie du pill in acute HIV-1 infection by detecting cell lesions; to detect anti HIV-1 activity of yu qin jie du pill by inhibition of P24 antigen on HIV-1Ⅲ_Bcells of chronic infection. Results： The 50% effective concentration（ EC50） of yu qin jie du pill inhibition of HIV-1Ⅲ_Binduced C8166 cells to form syncytia was 0. 035-0. 070 mg·mL^-1,treatment index was 42. 79 - 57. 03 mg·mL^-1; The 50% effective concentration（ EC50） of yu qin jie du pill inhibition of HIV-1 Ⅲ_Binfected C8166 P24 antigen expression was 0. 070 - 0. 090 mg · mL^-1,and the therapeutic index was22. 82 - 28. 51 mg·mL^-1. Conclusion： Yu qin jie du pill had certain anti-HIV-1 activity.