DS-1-47促着床的靶点分子筛选研究

Study and Screen out Possible Molecular Targets of DS-1-47 Promoting Embryo Implantation

目的研究筛选DS-1-47促着床的分子靶点。方法采用皮下注射吲哚美辛的方法建立胚泡着床障碍动物模型,动物随机分为正常组(N)、模型组(M)和中药组(Z),运用蛋白组学激光捕获显微切割技术-二维电泳-质谱(LCM-DE-MS)的技术路线研究分析DS-1-47作用前后子宫组织中蛋白分子的变化,筛选得到DS-1-47促进着床的关键分子,从而深入阐明其发挥作用的分子机制。结果 Z组能使11种蛋白质的表达发生明显改变,其中3种蛋白质的表达M组较N组明显下调,Z组较M组明显上调,这3种蛋白质经鉴定分别为肝羧酸酯酶、丝氨酸蛋白酶抑制剂、载脂蛋白A-Ⅰ;另外8种蛋白质的表达M组较N组明显上调,Z组较M组明显下调,蛋白质分别为血液结合素、激肽原-1、丝氨酸蛋白酶抑制剂A3K、α-1-抗胰蛋白酶1-3、结合珠蛋白、延伸因子-1-Δ、膜联蛋白A5、血清淀粉样蛋白P成分。结论血液结合素、结合珠蛋白、载脂蛋白A-Ⅰ、膜联蛋白A5、激肽原-1、延伸因子-1-Δ、丝氨酸蛋白酶抑制剂等可能是复方DS-1-47促进着床的重要靶点。

Objective To study and screen out possible molecular targets of DS-1-47 promoting embryo implantation.Methods Subcutaneous injection of indomethacin was used to establish the model of embryo implantation dysfunction.Animals were randomly divided into normal group（N）,model group（M）,and traditional Chinese medicine group（Z）;Using the technique of proteomics laser capture microdissection-double dimension electrophoresis-mass spectum（LCM-DE-MS）to analyze the protein molecules that were regulated by DS-1-47 in uterine tissue,and to screen the key molecules in promoting implantation,so as to further clarify the molecular mechanism and function targets of the drug.Results Totally there were 11 proteins expression was regulated obviously,including 8 proteins which were up-regulated in M group but down-regulated in Z group and were identified as hemopexin,kallikrein-1,serine protease inhibitor A3 K,α-1-antitrypsin1-3,haptoglobin,elongation factor-1-Δ,membrane associated protein A5,serum amyloid P-component;3 proteins which were down-regulated in M group but up-regulated in the Z group and were identified as liver acid esterase,serine protease inhibitor,and apolipoprotein A-Ⅰ.Conclusion Hemopexin,haptoglobin,serine protease inhibitor,apolipoprotein A-Ⅰ,kallikrein-1,elongation factor-1-Δ,and membrane associated protein A5 may be important targets for DS-1-47 to promote implantation.