黄芩苷防治雏鸡传染性支气管炎的药理作用

Pharmacology of prevention and control of avian infectious bronchitis by baicalin

为探讨黄芩苷对雏鸡感染传染性支气管炎M41(IBV-M41)的防治效果,将150只12日龄白羽肉鸡随机均分为10组,分别为黄芩苷高、中、低剂量预防组,黄芩苷高、中、低剂量治疗组,双黄连对照组,麻杏石甘颗粒对照组,模型对照组,空白对照组。除空白对照组外其余各组每只鸡于15日龄接种IBV-M41 0.2 ml。记录雏鸡的采食量及体质量变化、发病情况,测定其生产性能指标、淋巴细胞增殖能力、淋巴细胞亚群及细胞因子浓度。结果表明攻毒后黄芩苷预防和治疗各组料质量比均显著或极显著低于模型对照组;黄芩苷预防高剂量组保护率较高,黄芩苷治疗组治愈率最高可达80%;黄芩苷预防和治疗组淋巴细胞转化能力有明显提升;黄芩苷可以显著提高感染雏鸡CD3+CD8+淋巴细胞比率,使IFN-α浓度长时间维持高水平,并且使CD4+/CD8+的比值及IL-4浓度维持一定的动态平衡。多项检测结果表明黄芩苷抗雏鸡感染IBV-M41兼具预防与治疗作用,其中预防以高剂量[42mg/(kg·d)]效果显著,治疗以中剂量[28 mg/(kg·d)]效果最佳。

To probe into the preventive and control effect of baicalin on avian infectious bronchitis,one hundred and fifty 12-day-old white broiler chickens were randomly divided into 10 groups which were prevention groups with baicalin at high,medium and low dosage,treatment groups with baicalin at a high,medium and low dosage,shuanghuanglian control group,maxingshigan particles control group,model and blank control group. The chickens were infected with avian infectious bronchitis virus M-41 except for those in blank control group. The intake and weight of chickens were recorded and their clinical symptoms were observed. The production performance,lymphocyte proliferation and the concentration of cytokines were detected. The feed/gain ratios of chichens in baicalin-treated groups were lower than those in model control group. The protective rates were high in prevention groups with baicalin at a high dosage and the cure rate was up to 80% in the treatment groups. The lymphocyte proliferation was promoted significantly in baicalin-treated groups. Baicalin enhanced the percentages of CD3+and CD8+,maintained a high level of IFN-α for a long time,and kept a dynamic balance of CD4+/CD8+and IL-4 concentraion. In conclusion,baicalin is capable of prevention and control of avian infectious bronchitis, and a moderate dose at [28 mg/( kg·d) ] for cure and a high dose at [42 mg/( kg·d) ] for prevention were recommended.