山茶油中茶皂素的分离及其性能分析

Separation of tea saponin from oil-tea camellia seed oil and its performance

从山茶油中分离出茶皂素,采用单因素实验和响应面实验对提取工艺进行优化,同时对提取的茶皂素性能进行分析,并与粕中茶皂素进行对比。结果表明,茶皂素最优提取工艺条件为:以无水乙醇为提取剂,液料比11∶1,提取时间1.25 h,提取温度60℃。在最优条件下,茶皂素的提取率为(82.50±0.03)%。经红外光谱分析,油中茶皂素结构与粕中茶皂素结构存在一些差异,且油中茶皂素的起泡性、泡沫稳定性和表面活性均强于粕中茶皂素。

Tea saponin was separated from oil-tea camellia seed oil. The extraction process was optimized by single factor experiment and response surface methodology,and the performance of the extracted tea saponin was analyzed and compared with that extracted from oil-tea camellia seed meal. The results showed that the optimal extraction conditions of tea saponin were obtained as follows： with anhydrous ethanol as extractant,ratio of solvent to material 11 ∶ 1,extraction time 1. 25 h and extraction temperature60 ℃. Under the optimal conditions,the extraction rate of tea saponin was（ 82. 50 ± 0. 03） %. IR results showed that tea saponins from oil-tea camellia seed oil and oil-tea camellia seed meal had some differences in structure. The foamability,foam stability and surface activity of tea saponin from oil-tea camellia seed oil were better than those of tea saponin from oil-tea camellia seed meal.