对叶百部遗传多样性的ISSR分析

ISSR analysis on genetic diversity of traditional medicinal plant Stemona tuberosa

目的分析不同居群对叶百部Stemona tuberosa的亲缘关系和遗传多样性。方法应用ISSR分子标记技术研究长江流域以南各省对叶百部的遗传多样性;采用POPGENE32进行亲缘关系分析,NTSYSpc-2.10E进行UPGMA聚类分析。结果 7条ISSR引物共检测到74个清晰的扩增位点,多态性位点74个,多态性位点百分率100%;有效等位基因数(Ne)为1.524 4,平均Nei’s基因多样性指数(He)为0.314 6,平均Shannon多样性指数(I)为0.478 6,各居群间的遗传距离介于0~0.950 2,平均值为0.416 3。结论从分子角度揭示了对叶百部具有较高的遗传多样性,其居群间亲缘关系与地理环境有一定的相关性,实验结果将为后续对叶百部引种栽培及根用药植物资源的持续利用,特异生物碱的筛选及其运用于化学分类,以及对叶百部道地药材产区的科学选择奠定理论基础。

Objective To analyze the relationship and genetic diversity of Stemona tuberosa in different populations. Methods ISSR molecular technique was be used to decipher the genetic diversity of S. tuberosa in South of the Yangtze River, The genetic relationships among different populations were analyzed based on software POPGEN32 and the DNA molecular dendrogram was structured according to the software NTSYSpc-2.10 E. Results Seventy-four obvious bands of different S. tuberosa populations were amplified with seven suitable ISSR primers, 74 of them were polymorphic sites, and the percentage of polymorphism bands reached to 100%; Effective number of alleles（Ne） is 1.524 4, the average Nei＇s genetic diversity（He） index is 0.314 6, the average Shannon＇s information index is 0.478 6, the genetic distance among populations is 0—0.950 2, and the average distance is 0.416 3. Conclusion The study revealed that a relative high genetic diversity occurred in different populations of S. tuberosa, the genetic variation is related to geographical distance in different populations of S. tuberosa. The study will throw light on how to introduce and cultivate traditional Chinese medicinal plant, how to screen peculiar pyrrolidine alkaloids from S. tuberosa, and how to build to scientific standards and theoretical basis for traditional geo-authentic crude drug in China.