野菊花的质量标准研究

Quality standard of Chrysanthemum indicum

目的以黄酮类化合物蒙花苷和倍半萜类化合物豚草素A为指标成分,对不同产地的野菊花药材进行定性定量研究,提高野菊花的质量标准。方法采用薄层色谱法(TLC)对蒙花苷和豚草素A进行定性分析;采用高效液相法(HPLC)测定蒙花苷和豚草素A的量;YMC C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈(A)-0.05%磷酸水溶液(B);梯度洗脱,0~18 min,25%~26%A;18~26 min,26%~32%A;26~33 min,32%~34%A;33~35 min,34%~40%A;35~65 min,40%~50%A;体积流量0.8 m L/min;检测波长203、340 nm;柱温35℃。结果蒙花苷和豚草素A的TLC鉴别特征较为明显,不同产地二者的量有所不同,其中信阳野菊花药材中蒙花苷和豚草素A的量最高,分别为6.53%和0.81%。结论首次建立了野菊花药材不同类指标成分的TLC和HPLC法测定综合评价方法,该方法简单、准确、重复性好,研究表明蒙花苷和豚草素A的量能够反映野菊花药材的品质,为提高野菊花现有的质量标准提供了科学的实验依据。

Objective To improve the quality standard of Flos Chrysanthemum Indicum from diffrent origins by analyzing on linarin and cumambrin A qualitatively and quantitatively. Methods Qualitative analysis of linarin and cumambrin A was carried out by thin layer chromatography（TLC）; Content determination of linarin and cumambrin A was using high performance liquid chromatography（HPLC） on YMC C_（18） column（250 mm × 4.6 mm, 5 μm）; The mobile phase was a mixture of acetonitrile-0.05% phosphoric acid solution; The elution mode was gradient system（0—18 min, 25%—26% A; 18—26 min, 26%—32% A; 26—33 min, 32%—34% A; 33—35 min, 34%—40% A; 35—65 min, 40%—50% A）; The flow rate was 0.8 m L/min; The detection wavelengths were 203 nm and 340 nm; The column temperature was 35 ℃. Results Linarin and cumambrin A by TLC was obvious. The concents of linarin and cumambrin A in Flos Chrysanthemum Indicum from diffrent origins were different. The concents of linarin and cumambrin A from Xinyang were the highest（6.53% and 0.81% respectively）. Conclusion It is the first time to establish a method to evaluate different components in Flos Chrysanthemum Indicum by TLC and HPLC. The method is simple, accurate and reproducible, which can effectively improve the existing quality standard of Flos Chrysanthemum Indicum. The result also showed Linarin and cumambrin A could reflect the quality of Flos Chrysanthemum Indicum.